Thin film Poly(piperazine-amide) composite membranes using sequential interfacial polymerization with tuning by Cu2+ have brought significant findings in it. The hydrophobicity is relatively enhanced for the copper containing membranes. The membrane in which copper solution is applied prior to piperizine (Memb-III) exhibits higher hydrophobicity where as membrane (Memb-II) in which copper solution is applied following piperizine, possesses higher roughness compared to other two. Filtration experiments in terms of salts, mono/disaccharides and hexaconazole indicate that modified membranes are of different behaviours according to their sequence of preparative methods. Memb-III has shown lower SO4=/C1(-) selectivity compared to Memb-II (i.e. 3.92), though they are in different range. The unmodified membrane (Memb-I) exhibits SO4=/Cl- selectivity 3.23 is in the same scale of Memb-III (2.27). Memb-III exhibits higher hexaconazole separation (91.5%) compared to Memb-II (i.e. 53.9%). The flux decline follows the order: field water > tap water > deionized water. The copper incorporated membrane (Memb-II) has shown a low flux decline compared to Memb-III as well as Memb-I. The antibacterial properties towards E. Coli and Bacillus subtilis are well reflected. The copper containing membranes have promising antibacterial properties and follows the order Memb-II > Memb-III Memb-I.