In the current work the Supercritical Anti-solvent Fractionation (SAF) methodology was applied to conventional extracts from Artemisia absinthium L (wormwood). Artemetin and casticin, two compounds found in wormwood extracts and with high structural similarity, were tracked in all the experiments. A Response Surface Methodology (RSM), based on Central Composite Design (CCD) was used for both the experimental design and the results correlation. Studied variables were pressure (from 8.0-15.0 MPa) and CO2 flow rate (from 10 to 60 g.min(-1)), while temperature (40 degrees C) and feed solution flow rate (0.45 mL.min (-1)) were maintained constant. Overall achieved yields were around 70%, being downstream vessel yields much higher than those in the precipitation vessel. The conditions predicted to reach an optimal overall yield and fractionation were 80 MPa and 10 g.min -1 (composite desirability = 0.7443). Casticin and artemetin were mainly obtained in the downstream vessel, being this fraction more enriched in artemetin than casticin. (C) 2019 Elsevier B.V. All rights reserved.