Separation Science and Technology, Vol.54, No.14, 2283-2293, 2019
Effect of the solid-liquid extraction solvent on the phenolic content and antioxidant activity of three species of Stevia leaves
In our study, it was found that solid-liquid extraction solvent influenced the phenolic content in leaf extracts of Stevia origanoides, S. ovata and S. viscida. The highest total phenolic and flavonoids contents, the highest scavenging activity of 2,2-diphenyl-1-picrylhydrazyl (DPPH center dot) radical, and the highest total antioxidant capacity values were observed when 50% ethanol was used. The phenolic compounds identi?ed in the Stevia leaf extracts by high-performance liquid chromatography with diode array detection (HPLC-DAD) include phenolic acids such as chlorogenic, caffeic, p-coumaric and vanillic acid, as well as flavonoids such as quercetin and kaempferol.
Keywords:Scavenging activity;phenolic compounds;high-performance liquid chromatography with diode array detection (HPLC-DAD);antioxidant capacity;Stevia