Oxidized Alginate Supplemented Gelatin Hydrogels for the In Situ Formation of Wound Dressing with High Antibacterial Activity

Thai Thanh Hoang Thi; Yunki Lee; Phuong Le Thi; Ki Dong Park

Macromolecular Research, Vol.27, No.8, 811-820, August, 2019

DOI10.1007/s13233-019-7115-3 Export Citation

Oxidized Alginate Supplemented Gelatin Hydrogels for the In Situ Formation of Wound Dressing with High Antibacterial Activity

Thai Thanh Hoang Thi, Yunki Lee¹, Phuong Le Thi¹, and Ki Dong Park^{1, †}

Faculty of Applied Sciences, Ton Duc Thang University, Ho Chi Minh City, Vietnam
¹Department of Molecular Science and Technology, Ajou University, Suwon 16499, Korea

E-mail:

The development of antibacterial wound dressing with easily removable characteristic and highly exudate absorption is becoming a major requirement in the treatment of chronic wounds. In this study, the in situ forming gelatin/oxidized alginate-tyramine hydrogels (GH/OATA) were designed to overcome the current limitations. These hybrid hydrogels were quickly and controllably formed from several seconds to a few minutes by horseradish peroxidase/H2O2. The hybrid hydrogels at the optimal composition of GH/OATA 50/50 v/v showed the unique feature including stiffness being similar to skin, high swelling ratio due to the hydrophilic property of oxidized alginate, low adhesive strength allowing for the easy dressing removal without pain. In addition, the hybrid hydrogels could release the H2O2 amount sustainably to kill bacteria. Furthermore, 3D culture of human dermal fibroblasts inside hybrid hydrogels achieved more than 80% cell proliferation which implies the cell-compatibility.

Keywords:wound dressing;in situ forming hydrogels;gelatin;alginate;antibacterial ability

[References]

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Schultz DMG, Romanelli M, Claxton K, Wound Repair. Regen., 13, S1 (2005)
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Seliktar D, Science, 336(6085), 1124 (2012)
Lih E, Lee JS, Park KM, Park KD, Acta Biomater., 8, 3261 (2012)
Lee Y, Bae JW, Oh DH, Park KM, Chun YW, Sung HJ, Park KD, J. Mater. Chem. B, 1, 2407 (2013)
Thi TTH, Lee JS, Lee Y, Park KM, Park KD, Macromol. Biosci., 16, 334 (2016)
Yoon DS, Lee Y, Ryu HA, Jang Y, Lee KM, Choi Y, Choi WJ, Lee M, Park KM, Park KD, Lee JW, Acta Biomater., 38, 59 (2016)
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Moura LI, Dias AM, Carvalho E, de Sousa HC, Acta Biomater., 9, 7093 (2013)
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Zhao NDHH, Pharm. Res., 8, 400 (1999)
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Bradley LC, Bade ND, Mariani LM, Turner KT, Lee D, Stebe KJ, ACS Appl. Mater. Interfaces, 9, 27409 (2017)
Bae JW, Choi JH, Lee Y, Park KD, J. Tissue Eng. Regen. Med., 9, 1225 (2014)
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[Referenced By]

[1] Dreifke MB, Jayasuriya AA, Jayasuriya AC, Mater. Sci. Eng. C-Biomimetic Supramol. Syst., 48, 651 (2015)

[2] Schultz DMG, Romanelli M, Claxton K, Wound Repair. Regen., 13, S1 (2005)

[3] Ghobril C, Charoen K, Rodriguez EK, Nazarian A, Grinstaff MW, Angew. Chem.-Int. Edit., 52, 14070 (2013)

[4] Konieczynska MD, Ghobril C, Perez-Viloria M, Tevis KM, Blessing WA, Nazarian A, Rodriguez EK, Grinstaff MW, Angew. Chem.-Int. Edit., 55, 9984 (2016)

[5] Seliktar D, Science, 336(6085), 1124 (2012)

[6] Lih E, Lee JS, Park KM, Park KD, Acta Biomater., 8, 3261 (2012)

[7] Lee Y, Bae JW, Oh DH, Park KM, Chun YW, Sung HJ, Park KD, J. Mater. Chem. B, 1, 2407 (2013)

[8] Thi TTH, Lee JS, Lee Y, Park KM, Park KD, Macromol. Biosci., 16, 334 (2016)

[9] Yoon DS, Lee Y, Ryu HA, Jang Y, Lee KM, Choi Y, Choi WJ, Lee M, Park KM, Park KD, Lee JW, Acta Biomater., 38, 59 (2016)

[10] Lee Y, Choi KH, Park KM, Lee JM, Park BJ, Park KD, ACS Appl. Mater. Interfaces, 9, 16890 (2017)

[11] Thi TTH, Lee JS, Lee Y, Park HB, Park KM, Park KD, ACS Macro Letters, 6, 83 (2017)

[12] Thi TTH, Lee Y, Ryu SB, Sung HJ, Park KD, RSC Adv., 7, 34053 (2017)

[13] Stephen-Haynes J, J., Nursing Residential Care, 17, 1 (2015)

[14] Moura LI, Dias AM, Carvalho E, de Sousa HC, Acta Biomater., 9, 7093 (2013)

[15] Chen H, Xing X, Tan H, Jia Y, Zhou T, Chen Y, Ling Z, Hu X, Mater. Sci. Eng. C-Biomimetic Supramol. Syst., 70, 287 (2017)

[16] Augst AD, Kong HJ, Mooney DJ, DJ, Macromol. Biosci., 6, 623 (2006).

[17] Koehler J, Wallmeyer L, Hedtrich S, Goepferich LM, Brandl FP, Macromol. Biosci., 17, 160036 (2017)

[18] Zhao NDHH, Pharm. Res., 8, 400 (1999)

[19] Ghobril C, Grinstaff MW, Chem. Soc. Rev., 44, 1820 (2015)

[20] Bradley LC, Bade ND, Mariani LM, Turner KT, Lee D, Stebe KJ, ACS Appl. Mater. Interfaces, 9, 27409 (2017)

[21] Bae JW, Choi JH, Lee Y, Park KD, J. Tissue Eng. Regen. Med., 9, 1225 (2014)

[22] Lee Y, Bae JW, Thi TTH, Park KM, Park KD, Chem. Commun., 51, 8876 (2015)

[23] Thi TTH, Lee Y, Ryu SB, Nguyen DH, Park KD, Biopolymers, 109, 109:e2 (2018)

[24] Molin HNSO, Dolonius L, Hansson HA, J. Histochem. Cytochem., 26, 1053 (1978)

[25] Schone L, Herrmann H, Atmospheric Chem. Phys., 14, 4503 (2014)

[26] Discher DE, Mooney DJ, Zandstra PW, Science, 324, 1673 (2009)

[27] Yang Y, Wang L, Yan F, Xiang X, Tang Y, Zhang L, Liu J, Qiu L, J. Ultrasound Med., 37, 2507 (2018)

[28] Hou J, Li C, Guan Y, Zhang Y, Zhu XX, Polym. Chem., 6, 2204 (2015)

[29] Chen W, Wang R, Xu T, Ma X, Yao Z, Chi B, Xu H, J. Mater. Chem. B, 5, 5668 (2017)

[30] Huang KT, Fang YL, Hsieh PS, Li CC, Dai NT, Huang CJ, J. Mater. Chem. B, 4, 4206 (2016)

[31] Xu W, Song Q, Xu JF, Serpe MJ, Zhang X, ACS Appl. Mater. Interfaces, 9, 11368 (2017)

[32] Lee SH, Lee Y, Chun YW, Crowder SW, Young PP, Park KD, Sung HJ, Adv. Funct. Mater., 24(43), 6771 (2014)

[33] Lee Y, Bae JW, Lee JW, Suh W, Park KD, J. Mater. Chem. B, 2, 7712 (2014)

[34] Morimoto HKK, Yabuta T, Iwanaga K, Kakemi M, Tabata Y, Ikada Y, Eur. J. Pharm. Sci., 13, 179 (2001)

[35] Smart JD, Adv. Drug Deliv. Rev., 57, 1556 (2005)