This study investigated methane production in an anaerobic sequencing batch biofilm reactor (AnSBBR) by co-digesting sugarcane vinasse and cheese whey. The assessment was based on the influence of feed strategy, interaction between cycle time and influent concentration, applied volumetric organic load (OLRA), and temperature over system stability and performance. The system showed flexibility with regard to the feed strategy, but the reduction of cycle time and influent concentration, at the same OLRA, resulted in lower methane productivity. Increasing organic load, up to the value of 15.27 gCOD L-1 day(-1), favored the process, increasing methane yield and productivity. Temperature reduction from 30 to 25 degrees C resulted in worse performance, although increasing it to 35 degrees C provided similar results to 30 degrees C. The best results were achieved at an OLRA of 15.27 gCOD L-1 day(-1), cycle time of 8 h, fed-batch operation, and temperature of 30 degrees C. The system achieved soluble COD removal efficiency of 89%, methane productivity of 208.5 molCH(4) m(-3) day(-1) and yield of 15.76 mmolCH(4) gCOD(-1). The kinetic model fit indicated methanogenesis preference for the hydrogenotrophic route. At the industrial scale estimative, considering a scenario with a sugarcane ethanol plant with ethanol production of 150,896 m(3) year(-1), it was estimated energy production of 25,544 MWh month(-1).