Bacteria induced infectious are one of the major problems during wound healing process. The overuse of antibiotics has resulted in the spread of resistant bacteria. In this work, Ag-doped TiO2 (Ag/TiO2) nanoparticles were embedded in PVA hydrogel to combat antibiotic-resistant bacteria efficiently by light-induced reactive oxygen species (ROS). The hybrid hydrogel was prepared via a simple two-step process. Ag/TiO2 nanoparticles were obtained via chemical reduction process followed by Ag/TiO2 nanoparticles were dispersed in PVA and freeze-thaw cycles five times to fabricate Ag/TiO2-incorporated hybrid hydrogel. The hydrogel exhibited excellent antibacterial activities against both Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus) under irradiation of 660 nm visible light (VL) within 5 min due to the release of ROS by photodynamic action. The hydrogel also possessed excellent biocompatibility. In addition, the hydrogel could efficiently prevent the S. aureus-accompanied wound infection in vivo and significantly accelerate wound healing in rat model compare with traditional 3M dressing.