Objectives To explore potential effects of recombinant peptidyl-prolyl isomerase human Cyclophilin A (CypA) in the growth of mouse hair and human hair follicle dermal papilla cells (HDP). Results The hair growth of 8-weeks C57BL/6 mouse was significantly stimulated by recombinant human CypA protein. CypA also promoted Human Dermal Papilla (HDP) cell growth and induced beta-catenin protein through GSK3 beta inhibition. Conclusions The molecular chaperone CypA stimulates hair follicle and HDP cells through Wnt/beta-catenin signaling pathway, suggesting that it might be a key molecule for hair loss disorder therapy.