Aerosol deposition is a unique spray coating process to deposit dense ceramic films directly at room temperature just by spraying a dry powder, without any binder or additional sintering step needed. Therefore, it is a very promising coating method for functional materials that may be used to produce a variety of electrochemical devices, solid oxide fuel cells or gas sensors. However, functional properties like the electrical conductivity significantly depend on the microstructure of the produced film. Consequently, anisotropic film properties may occur even for isotropic functional materials. In this work, films of oxide ion-conducting yttria-stabilized zirconia (YSZ) were produced by aerosol deposition. The orientation-dependent electrical conductivity was measured using two different electrode configurations: one for the in-plane conductivity and another one for through-plane measurements. A slight anisotropy was observed, attributed to the present film morphology. In particular, the grain boundaries influenced the total conductivity. Furthermore, a thermal post-deposition treatment significantly affected the total conductivity of aerosol-deposited YSZ films.