A novel electrochemical detection platform was constructed based on dewdrop-like platinum nanoparticles-decorated silver nanoflowers nanocomposites coated with bovine serum albumin (AgNFs-Pt@BSA), which were synthesized through replacement reaction between chloroplatinic acid and AgNFs. As expected, in final obtained AgNFs-Pt@BSA nanocomposites, the petal structures of AgNFs with broad surface areas led to a high electron conductivity; Pt nanoparticles favorably enhanced the electrocatalytic ability toward hydrogen peroxide (H2O2); bovine serum albumin (BSA) coated around AgNFs-Pt provided numerous functional groups (especially-NH2). Inspired by above characteristics, a remarkable glucose biosensor could be assembled through crosslinking glucose oxidase with AgNFs-Pt@BSA via glutaraldehyde (AgNFs-Pt@BSA/GA/GOD). The high electrochemical responses were exhibited linearly in a wide concentrate range of glucose from 1 to 14 mM with a low detection limit of 0.3 mM at a signal-to-noise ratio of 3. Furthermore, with the advantages of favorable selectivity, excellent reproducibility and durable stability, here prepared electrochemical sensor showed promising analytical performance in the detection of glucose in human serum samples. All satisfied results provided a considerable direction of advance and prospect for the clinical applications of human blood glucose detection in the coming future. (C) 2019 The Electrochemical Society.