Now a day, silver nanoparticles (AgNps) have attracted considerable attention in various biomedical applications like drug delivery, photo thermal ablation of cancer and imaging agent for cancer diagnosis. The present work is demonstrated on the nano silver embedded soy protein polyacrylamide (PAM-SP@Ag) nanocomposite hydrogel which is synthesized by in situ polymerization process for the release of ciprofloxacin drugs. The structure and morphology of the nanocomposite hydrogels have been explored using FTIR, XRD, XPS, and FESEM analysis. It is found that, the average size of AgNps is 50 nm whereas; size distributions of AgNps are studied from DLS study. The investigation of the release rate of ciprofloxacin drugs carried out by measuring water retention and swelling properties of nanocomposite hydrogels. The in vitro ciprofloxacin drug release rate is noticed to be 95.3% in a time period of 6hr. The investigation of cytotoxicity tests and antibacterial behaviour along with negative and positive action of nanocomposite hydrogels are studied. The gelling actions of prepared nanocomposite hydro gels are given by the rheological study. The cytotoxicity behaviour of PAM-SP@Ag nanocomposite hydrogels along with improved swellings and rheological properties are enabling the material as suitable vehicle for safe release of ciprofloxacin drug.