The transient behavior of enzyme-catalyzed reaction in a proposed chromatographic reactor was investigated, and application of the technique to evaluate the capability of the enzyme in the process of kinetic resolution was developed. The theoretical result reveals that the enantioselectivity of the enzyme can be obtained directly from the amount of substrates eluted without any further treatment if a large amount of enzyme is provided. Because of its simplicity in operation and data analysis yet without any adverse consequences induced by enzyme deactivation, the technique presented can facilitate the development of an optimal condition for enzymatic kinetic resolutionl.