Biotechnology Letters, Vol.42, No.1, 67-78, 2020
Expression of T9W in Pichia pastoris and the protective roles of T9W in ICR Mice
Background The purpose of this paper is to express the novel alpha-helical peptide T9W more efficiently using the Pichia pastoris expression system and to examine the role of T9W in ICR mice. Results The novel antimicrobial peptide T9W was expressed in P. pastoris X-33 by using the vector pPICZ alpha A. Approximately 13 mg/L T9W was secreted from the culture and purified. The expressed peptide has similar activity to the synthetic peptide. ICR female mice challenged with P. aeruginosa 27853 at the LD100 were treated with T9W and CPFX. The results showed that the secretion of inflammatory cytokines and lung damage was significantly reduced by the treatment group, and the protective response was equivalent between T9W and ciprofloxacin-treated mice. Conclusion T9W was expressed in P. pastoris X-33 via the methanol-inducible vector pPICZ alpha A and exhibited the same biological activity as synthetic T9W. T9W can alleviate damage to mice caused by P. aeruginosa.