The identifiability of the fundamental kinetic parameters of a model of a plug-flow isotope transient tracing of methanol over PdNO/Al was investigated. The values of the parameters were estimated by securing a least-squares fit between the tracer data and the partial differential equations describing the reaction response in a plug-flow mode. The results demonstrate that modeling and identifiability of isotope transient tracing data provide for a meaningful assessment of heterogeneous catalytic reaction systems.