An integrated process for bioethanol production from Miscanthus sacchariflorus was used to construct a bench-scale plant constructed and an economic analysis was carried out to investigate the feasibility of its application to a commercial plant. The bench-scale plant was operated for 1 month and an economic analysis and sensitivity analysis was performed on the data acquired. In this study, 100,000 kL of bioethanol could be produced annually from 606,061 tons of M. sacchariflorus and the production cost was calculated to be USS1.76/L. However, the by-products of this process such as xylose molasses and lignin can be sold or used as a heat source, which can decrease the ethanol production costs. Therefore, the final ethanol production cost was calculated to be USS1.31/L, and is considerably influenced by the enzyme cost. The results and data obtained should contribute to the development of a commercial-scale lignocellulosic bioethanol plant.