화학공학소재연구정보센터
홈 로그인 로그아웃 연락처 사이트맵
Macromolecular Research, Vol.28, No.4, 390-399, April, 2020
DOI10.1007/s13233-020-8051-y  Export Citation
Development of Molecularly Imprinted Membranes for Selective Determination of Urinary Ultra-Trace 5-Fluorouracil as Antineoplastic Drug Used in Chemotherapy
Zahra Beigzadeh1, Farideh Golbabaei1, Monireh Khadem1, Fariborz Omidi2, Mirghani Seyed Someah1, and Seyed Jamaleddin Shahtaheri1, 3, †
  • 1Department of Occupational Health Engineering, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
  • 2Research Center for Environmental Determinants of Health (RCEDH), Health Institute, Kermanshah University of Medical Sciences, Kermanshah, Iran
  • 3Institute for Environmental Research, Tehran University of Medical Sciences, Tehran, Iran
E-mail:
Background: The incidence of neoplasm has significantly increased around the world over the last decades, resulting in use of anti-neoplastic drugs and the potential for occupational exposure to these drugs. One of the most commonly used chemotherapeutic drugs for treatment of malignancies is 5-fluorouracil (5-FU). Objectives: In this study, in order to develop an appropriate adsorbent for selective extraction of ultra-trace 5-fluorouracil from urine samples, molecularly imprinted membranes (MIMs) of 5-FU were prepared by encapsulating molecularly imprinted polymer nanoparticles (MIP-NPs) into the electrospun polyethylene terephthalate (PET) nanofibers. Methods: The MIP-NPs encapsulated into electrospun PET nanofibers were prepared by precipitation polymerization technique. These MIMs were used as selective sorbents for solid-phase extraction (SPE) of ultra-trace 5-FU from different samples prior to the analysis by high performance liquid chromatography (HPLC). Central Composite Design (CCD) was used for optimization of effective parameters in sorption and desorption steps of the molecularly imprinted membrane solid phase extraction (MIMSPE). Results: The optimum operating conditions for the proposed method were pH: 6.2, amount of sorbent: 10.3 mg, and absorption time: 20.5h, the volume of eluent: 1 mL, the composition of elution solvent: the ratio of 90.3:9.7 methanol and acetic acid, and desorption time: 1.27 h. Under these optimized conditions, the limit of detection for 5-FU by MIMSPE was 0.07 μg L-1. The recoveries of 5-FU from urine sample ranged from 92.95±3.85 to 94.85±2.85%. Conclusion: The proposed MIMSPE-HPLC method can potentially be applied for selective detection of 5-FU in real samples with no special sample pretreatment steps.
Keywords:molecularly imprinted membrane;electrospinning;molecularly imprinted membrane solid phase extraction;central composite design;5-fluorouracil;antineoplastic drug
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