A multiphase second-order moment turbulent model was developed including an improved closure Reynolds stress transport to describe effectively the anisotropic characteristics of bubble-liquid turbulent flow. The two-phase hydrodynamics in a bubble column were numerically simulated by an in-house code. The effect of the amount of jetting holes on bubble-liquid hydrodynamics in relationship with a cell culture was investigated. Firstly, the bubble energy production correlation was proposed to verify the higher cell damage rate near gas inlet regions. The larger amounts of jetting holes resulted in an increase of bubble stress, bubble kinetic energy, and energy dissipation, particularly bubble energy production at the near gas inlet, which boosted cell damage.