In this study, a new nano-sized mesoporous silica (MSN) as support for Ni-based catalysts was produced from natural resources and tested in the dry reforming of methane between 823 and 1023 K. The fresh and spent catalysts Ni-x/MSN (x = 5, 10 and 20 wt.%) were characterized by various techniques. All catalysts are selective for hydrogen production and exhibited long-term stability with low coke formation predominantly as carbon nanotubes, for Ni loadings less than 10% at 973 K. The catalytic results were correlated with the in situ generation of Ni nanoparticles which are highly dispersed on the MSN surface due to strong metal-support interactions thus preventing the sintering process. No significant deactivation was recorded along 25 h on stream meaning that the textural properties of the catalysts have not been altered by the coke deposition or reaction temperature. The prepared MSN is a potential support to be utilized for hydrogen generation. (C) 2018 Hydrogen Energy Publications LLC. Published by Elsevier Ltd. All rights reserved.