Improved biodegradation of polyvinyl alcohol by hybrid nanoflowers of degrading enzymes from Bacillus niacini

Hongjie Bian; Gaoyang Wang; Mengfei Cao; Ziyuan Wang; Jiandong Cui

Korean Journal of Chemical Engineering, Vol.37, No.6, 1020-1028, June, 2020

DOI10.1007/s11814-020-0547-9 Export Citation

Improved biodegradation of polyvinyl alcohol by hybrid nanoflowers of degrading enzymes from Bacillus niacini

Hongjie Bian^{1, 2}, Gaoyang Wang², Mengfei Cao¹, Ziyuan Wang², and Jiandong Cui^{2, †}

¹Research Center for Fermentation Engineering of Hebei, College of Bioscience and Bioengineering, Hebei University of Science and Technology, 26 Yuxiang Street, Shijiazhang 050000, P. R. China
²State Key Laboratory of Food Nutrition and Safety, Laboratory of Industrial Fermentation Microbiology, Ministry of Education, Tianjin University of Science and Technology, No 29, 13th, Avenue, Tianjin Economic and Technological Development Area (TEDA), Tianjin 300457, P. R. China

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Polyvinyl alcohol (PVA) is a synthetic polymer that is difficult to degrade in nature. In this study, we synthesized PVA-degrading enzymes (PVAase)-Cu3(PO4)2 hybrid nanoflowers by using crude PVAase from Bacillus niacini for PVA degradation. The influences of PVAase concentration, Cu2+ concentration, and incubation time on the nucleation and activity of the PVAase hybrid nanoflower were investigated. The maximal activity recovery of the PVAase hybrid nanoflower was approximately 85% at 0.25 mg/mL of PVAase, 0.36mM Cu2+, and 72 h incubation time. The optimum temperature and pH of PVAase did not change before and after immobilization. Compared with free PVAase, the PVAase hybrid nanoflower showed high thermal stability and storage stability. Additionally, the PVAase hybrid nanoflower displayed excellent reusability after eight cycles and promising PVA degradability, indicating its potential application in PVA degradation.

Keywords:Polyvinyl Alcohol;Biodegradation;Hybrid Nanoflower;Immobilization Enzyme;Purification

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[2] Halima NB, RSC Adv., 6, 39823 (2016)

[3] Liu YL, Deng YL, Chen P, Duan MJ, Lin XS, Zhang Y, J. Basic. Microbiol., 59, 368 (2019)

[4] Balasubramanian RK, Colloids Surf. A: Physicochem. Eng. Asp., 455, 174 (2014)

[5] Kim MN, Yoon MG, Polym. Degrad. Stabil., 95, 89 (2010)

[6] Stoica-Guzun A, Jecu L, Gheorghe A, Raut I, Stroescu M, Ghiurea M, Danila M, Jipa I, Fruth V, J. Polym. Environ., 19, 69 (2011)

[7] Chiellini E, Corti A, D’Antone S, Solaro R, Prog. Polym. Sci, 28, 963 (2003)

[8] Halima NB, RSC Adv., 6, 39823 (2016)

[9] Suzuki T, Ichihara Y, Yamada M, Tonomura K, Agric. Biol. Chem., 37, 747 (1973)

[10] Tsujiyama S, Nitta T, Maoka T, J. Biosci. Bioeng., 112(1), 58 (2011)

[11] Bian HJ, Cao MF, Wen H, Tan ZL, Jia SR, Cui JD, Int. J. Biol. Macromol., 124, 10 (2019)

[12] Ullah M, Weng CH, Li H, Sun W, Zhang H, Song AH, Zhu H, Environ. Technol., 39, 2056 (2018)

[13] Shimao M, Tamogami T, Kishida S, Microbiology, 146, 649 (2000)

[14] Wei YH, Fu J, Wu JY, Jia XY, Zhou YH, Li CD, Dong MX, Wang SS, Zhang J, Chen F, Appl. Eviron. Microbial., 84, e01898 (2018)

[15] Yang Y, Zhang DX, Liu S, Jia DX, Du GC, Chen J, J. Ind. Microbiol. Biotechnol., 39, 99 (2012)

[16] Jia DX, Li JH, Liu L, Zhang DX, Yang Y, Du GC, Chen J, Appl. Microbiol. Biotechnol., 97(3), 1113 (2013)

[17] Tang B, Liao XY, Zhang DX, Li M, Li R, Yan KL, Du GC, Chen J, Polym. Degrad. Stabil., 95, 557 (2010)

[18] Min L, Zhang DX, Du GC, Chen J, J. Microbiol. Biotechnol., 22, 220 (2012)

[19] Ren SZ, Li CH, Jiao XB, Jia SR, Jiang YJ, Bilal M, Cui JD, Chem. Eng. J., 373, 1254 (2019)

[20] Lin Z, Xiao Y, Wang L, Yin YQ, Zheng JN, Yang HH, Chen GN, RSC Adv., 4, 13888 (2014)

[21] Ge J, Lei JD, Zare RN, Nat. Nanotechnol., 7(7), 428 (2012)

[22] Lin Z, Xiao Y, Yin YQ, Hu WL, Liu W, Yang HH, ACS Appl. Mater. Interfaces, 6, 10775 (2014)

[23] Somturk B, Hancer M, Ocsoy I, Ozdemir N, DALTON T., 44, 13845 (2015)

[24] Zhang BL, Li PT, Zhang HP, Fan LL, Wang H, Li XJ, Tian L, Ali N, Ali Z, Zhang QY, RSC Adv., 6, 46702 (2016)

[25] Cui JD, Zhao YM, Liu RL, Zhong C, Jia SR, Sci. Rep., 6, 27928 (2016)

[26] Wu ZF, Li H, Zhu XJ, Li S, Wang Z, Wang L, Li ZQ, Ch Q, Catalyst, 7, 188 (2017)

[27] Qian K, Wang H, Liu JM, Gao ST, Liu WT, Wan X, Zhang YY, Liu QS, Yin XY, New J. Chem., 42, 429 (2018)

[28] Yu Y, Fei X, Tian J, Xu LQ, Wang XY, Wang Y, Colloids Surf. B: Biointerfaces, 130, 299 (2015)

[29] Zhao M, Rong JH, Han J, Zhou Y, Li CM, Wang L, Mao YL, Wang Y, ACS Appl. Mater. Interfaces, 11, 31878 (2019)

[30] Bradford MM, Anal. Biochem., 72, 248 (1976)

[31] Finley JH, Anal. Chem., 33, 1925 (1961)

[32] Escobar S, Velasco-Lozano S, Lu CH, Lin YF, Mesa M, Bernal C, Lopez-Gallego F, J. Mater. Chem. B, 5, 4478 (2017)

[33] Lee SW, Cheon SA, Kim MI, Park TJ, J. Nanobiotechnol., 13, 54 (2015)

[34] Yin UQ, Xiao Y, Lin G, Xiao Q, Lin Z, Cai ZW, J. Mater. Chem. B, 3, 2295 (2015)

[35] Altinkaynak C, Yilmaz I, Koksal Z, Ozdemir H, Ocsoy I, Ozdemir N, Int. J. Biol. Macromol., 84, 402 (2016)

[36] Zhang BL, Li PT, Zhang HP, Wang H, Li XJ, Tian L, Ali N, Ali Z, Zhang QY, Chem. Eng. J., 291, 287 (2016)

[37] Baldemir A, Kose NB, Ildiz N, Ilgun S, Yusufbeyoglu S, Yilmaz V, Ocsoy I, RSC Adv., 7, 44303 (2017)

[38] Rodrigues RC, Ortiz C, Berenguer-Murcia A, Torres R, Fernandez-Lafuente R, Chem. Soc. Rev., 42, 6290 (2013)

[39] Yabuki S, Catalyst, 7, 36 (2017)