In this paper, magnetic substrate, molecular imprinting technology (MIT) and surface-enhanced Raman scattering (SERS) technology were combined to prepare core-shell magnetic molecularly imprinted polymer Fe3O4@SiO2@Ag-MIPs for quantitative detection of ofloxacin. The results showed that the concentration of ofloxacin within the range of 10(-3)-10(-8) mol/L showed a good linear relationship with the Raman peak intensity at 1416 cm(-1). The prepared molecularly imprinted polymer had excellent uniformity and dispersibility. Raman testing of commercially available ofloxacin eye drops showed good recovery, indicated that it can be used for the quantitative detection of actual samples.