In this study, a wild-type Pseudomonas aeruginosa strain KT1115 with the capability of converting rapeseed oils into di-rhamnolipids, a class of biosurfactants with extensive application potential, was successfully isolated and characterized. Di-rhamnolipids production by microorganism culture provided a mild, eco-friendly, and secure approach for surfactants production instead of conventional chemical synthesis. However, few studies have been attempted to explore the metabolic mechanism behind the high di-rhamnolipids production by P. aeruginosa. Here, we presented the graft genome of a wild-type P. aeruginosa strain KT1115, with emphasis on the analysis of oils metabolism and rhamnolipid synthesis. The availability of the genome sequence provides additional insight into the genetic mechanism enhancing di-rhamnolipids biosynthesis.