Highly ordered nanotubes titanium dioxide (NTs-TiO2) array was fabricated and used as the support of Pt nanoparticles for the selective aqueous phase of glycerol (GLY) electrooxidation. The structure and morphology of the NTs-TiO2/Pt were characterized by X-ray diffraction, scanning electron microscope, transmission electron microscope, etc. The electrooxidation GLY properties of NTsTiO2/Pt were investigated by cyclic voltammetry, linear sweep voltammetry, and accelerated service life test. The results show that, compared with bulk Pt and nano-TiO2/Pt, the NTs-TiO2/Pt have the highest service life and electrooxidation activity and selection for GLY. At the reaction condition of 1.2 V potential, the NTs-TiO2/Pt catalyst afforded 67.1% selectivity for the glyceric acid.