Metal-organic frameworks (MOFs)-supported noble metal nanoparticles (NMNPs) catalysts attract increasing attention due to their high durability and efficiency in catalytic reactions. In this work, Pt nanoparticles are inserted at the designated location inside MIL-100(Fe) to investigate the location-effect of NMNPs inside MOFs on the catalytic reaction. The comparison test on the reduction of p-nitrophenol suggests that the location of the Pt nanoparticles inside MIL-100(Fe) largely influences the catalytic performance and the Pt nanoparticles inside MIL-100(Fe) locate closer to the outer surface of the MIL-100(Fe), providing higher catalytic efficiency. Possible reasons are concluded as the location of the Pt nanoparticles inside MIL-100(Fe) dominates the reactants transport pathway and the loading amount of Pt nanoparticles.