화학공학소재연구정보센터
홈 로그인 로그아웃 연락처 사이트맵
Korean Journal of Chemical Engineering, Vol.37, No.9, 1522-1529, September, 2020
DOI10.1007/s11814-020-0564-8  Export Citation
Simultaneous accumulation of lipid and carotenoid in freshwater green microalgae Desmodesmus subspicatus LC172266 by nutrient replete strategy under mixotrophic condition
Chijioke Nwoye Eze1, 2, †, Hideki Aoyagi2, and James Chukwuma Ogbonna1
  • 1Department of Microbiology, Faculty of Biological Sciences, University of Nigeria, Nsukka, Nigeria
  • 2Faculty of Life and Environmental Sciences, University of Tsukuba, Tsukuba, Japan
E-mail:
Production of biofuel by microalgae can become economically viable if other high-value commercial products such as carotenoid are simultaneously produced. This study aimed at enhancement of lipid and carotenoid productivity by an oleaginous microalga sp. Desmodesmus subspicatus LC172266 by supplementing Nitrogen sources to BG 11 medium under batch or fed batch mixotrophic condition. Optimum urea supplement (0.2 gL-1) to BG 11 medium enhanced carotenoid content of the D. subspicatus by about 3.7 fold (3.02±0.002mgg-1 cell) and 3.0 fold (3.86±0.001mgg-1 cell) using glucose or glycerol as organic carbon sources, respectively. Also, lipid productivity was enhanced by about 2.0 (0.07817±0.0028 gL-1day-1) (0.265±0.006 gg-1 cell) and 2.5 (0.08426±0.0008 gL-1day-1) (0.285± 0.02 gg-1 cell) fold. Fed-batch cultivation with glucose at a feed rate of 0.5 gL-1 per day enhanced both carotenoid and lipid productivities of D. subspicatus by 34% and 45%, respectively, after eight days of mixotrophic cultivation.
Keywords:Lipid Productivity;Desmodesmus subspicatus;Fed-batch Culture;Mixotrophic Culture;Carotenoid
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