Effect of CMC Concentration on Cell Growth Behavior of PVA/CMC Hydrogel

Ji-Yeon Shin; Deuk Yong Lee; Jung In Yoon; Yo-Seung Song

Macromolecular Research, Vol.28, No.9, 813-819, August, 2020

DOI10.1007/s13233-020-8106-0 Export Citation

Effect of CMC Concentration on Cell Growth Behavior of PVA/CMC Hydrogel

Ji-Yeon Shin, Deuk Yong Lee^†, Jung In Yoon¹, and Yo-Seung Song²

Department of Biomedical Engineering, Daelim University, Anyang 13916, Korea
¹HANBIO Co., Ltd., Seoul 06322, Korea
²Department of Materials Engineering, Korea Aerospace University, Goyang 10540, Korea

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Polyvinyl alcohol (PVA)/carboxymethyl cellulose (CMC) hydrogels containing various CMC concentration of 2 to 10 wt% are prepared using cyclic freezing/thawing and subsequent γ-ray irradiation to evaluate the effect of CMC content on cell growth rate of the hydrogels. A 3-D porous network structure is successfully formed via physical and chemical crosslinking. The pore size and the metronidazole drug release rate of the PVA/CMC hydrogels increase with raising the CMC concentration from 2 to 10 wt%, however, the structural integrity is severely damaged. The highest compressive strength of 99.2 ± 9.6 kPa and the fastest cell growth determined by scratch assay and enzyme-linked immunosorbent assay are observed for the irradiated PVA/6 wt% CMC hydrogels, indicating that they are highly suitable for wound dressing requiring fast regeneration.

Keywords:polyvinyl alcohol (PVA);sodium carboxymethyl cellulose (CMC);scaffold;freezing/thawing;γ-ray irradiation;cell growth;scratch assay;ELISA assay

[References]

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Shin J, Jeong H, Lee DY, J. Biomed. Eng. Res., 39, 161 (2018)
Lee MK, Bae H, Lee S, Chung N, Lee H, Choi S, Hwang S, Lee J, Macromol. Res., 19(2), 130 (2011)
Lee DY, Kim D, Kim B, Park JY, Lee J, J. Ceram. Proc. Res., 19, 15 (2018)
Jeong H, Rho J, Shin J, Lee DY, Hwang T, Kim KJ, Biomed. Eng. Lett., 8, 267 (2018)
Mahnama H, Dadbin S, Frounchi M, Rajabi S, Artif. Cell Nanomed. Biotechnol., 45, 928 (2017)
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Oh GY, Rho JW, Lee DY, Lee MH, Kim YZ, Macromol. Res., 26(1), 48 (2018)
Kim Y, Son S, Chun C, Kim J, Lee DY, Choi HJ, Kim T, Biomed. Eng. Lett., 6, 287 (2016)
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Baik J, Park J, Jeong J, Jeong S, Gwon H, Ahn S, Lim Y, J. Rad. Ind., 10, 21 (2016)
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[Referenced By]

[1] Han S, You H, J. Korean Med. Assoc., 54, 594 (2011)

[2] Shin J, Jeong H, Lee DY, J. Biomed. Eng. Res., 39, 161 (2018)

[3] Lee MK, Bae H, Lee S, Chung N, Lee H, Choi S, Hwang S, Lee J, Macromol. Res., 19(2), 130 (2011)

[4] Lee DY, Kim D, Kim B, Park JY, Lee J, J. Ceram. Proc. Res., 19, 15 (2018)

[5] Jeong H, Rho J, Shin J, Lee DY, Hwang T, Kim KJ, Biomed. Eng. Lett., 8, 267 (2018)

[6] Mahnama H, Dadbin S, Frounchi M, Rajabi S, Artif. Cell Nanomed. Biotechnol., 45, 928 (2017)

[7] Li Y, Zhu C, Fan D, Fu R, Ma P, Duan Z, Li X, Lei H, Chi L, Macromol. Biosci., 1800424 (2019).

[8] Thangprasert A, Tansakul C, Thuaksubun N, Meesane J, Mater. Des., 183, 108113 (2019)

[9] Kumar B, Sauraj, Negi YS, Mater. Lett., 252, 308 (2019)

[10] Ghorpade VS, Dias RJ, Mali KK, Mulla SI, J. Drug Deliv. Sci., 52, 421 (2019)

[11] Buhus G, Popa M, Peptu C, Desbrueres J, J. Optoelectron. Adv. Mater., 9, 3345 (2007)

[12] Jo SY, Lim YM, Youn MH, Gwon HJ, Park JS, Nho YC, Shin H, Polym. Korea, 33(6), 551 (2009)

[13] Dahlan NA, Pushpamalar J, Veeramachineni AK, Muniyandy S, J. Polym. Environ., 26, 5 (2017)

[14] Shin JY, Lee DY, Song YS, Kim BY, Polym. Korea, 42(6), 967 (2018)

[15] Oh GY, Rho JW, Lee DY, Lee MH, Kim YZ, Macromol. Res., 26(1), 48 (2018)

[16] Kim Y, Son S, Chun C, Kim J, Lee DY, Choi HJ, Kim T, Biomed. Eng. Lett., 6, 287 (2016)

[17] Kim J, Lee DY, Kim E, Janag J, Cho N, Tissue Eng. Regen. Med., 11, 32 (2014)

[18] Kim J, Lee DY, Kim T, Lee M, Cho N, Met. Mater. Int., 20, 555 (2014)

[19] Baik J, Park J, Jeong J, Jeong S, Gwon H, Ahn S, Lim Y, J. Rad. Ind., 10, 21 (2016)

[20] Alici E, Sutlu T, Bjorkstrand B, Gilljam M, Stellan B, Nahi H, Quezada HC, Gahrton G, Ljunggren HG, Dilber MS, Blood, 111, 3155 (2008)

[21] Morris RJ, Chong LK, Wilkinson GWG, Wang ECY, J. Immunol. Methods, 307, 24 (2005)

[22] Cho D, Campana D, Korean J. Lab. Med., 29, 89 (2009)

[23] Torelli GF, Rozera C, Santodonato L, Peragine N, Daqostino G, et al., Blood Transfus., 13, 464 (2015)

[24] Jonkman JEN, Cathcart JA, Xu F, Bartolini ME, Amon JE, Stevens KM, Colarusso P, Cell Adhes. Migr., 8, 440 (2014)

[25] El-Kased RF, Amer RI, Attia D, Elmazar M, Sci. Rep., 7, 1 (2017)

[26] Santos SC, Custodia CA, Mano JF, Adv. Healthc. Mater., 1800849 (2018).

[27] Xin X, Yang S, Ingle G, Zlot C, Rangell L, Kowalski J, Schwall R, Ferrara N, Gerritsen ME, Am. J. Pathol., 158, 1111 (2001)

[28] Noverina R, Widowati W, Ajuningtyas W, Kurniawan D, Afifah E, et al., Clin. Nutrition Exp., 24, 34 (2019)

[29] Sagaradze G, Grigorieva O, Nimiritsky P, Basalova N, Kalinina N, Akopyan Z, Efimenko A, Int. J. Mol. Sci., 20, 1656 (2019)

[30] Al-bermany AJ, Kadem BY, Kadouri LTH, Adv. Phys. Theor. Appl., 15, 11 (2013)