Aims The objective of this work was to identify a fungal strain showing potential biocontrol abilities against twoFusariumdamping-off agents and to test it as a Biological Control Agent (BCA) in maize seed coating under field conditions. Methods and Results A collection of native fungal strains associated with maize in Belgium was screened for antagonistic potential againstFusarium avenaceumandFusarium culmorum. The strain with highest biocontrol potential was identified as an endophyticTrichoderma atrovirideBC0584. In greenhouse, it significantly improves the emergence of seedlings infected byF. avenaceumorF. culmorumpathogens. In most field trials carried out during the season 2017, it significantly increased the emergence rate of infected seedlings compared to untreated seeds. One slurriable powder formulation allows BCA conidia to survive over a 6-month storage period at 4 degrees C. Conclusions The fungal BC0584 strain is a promising BCA that could be an alternative to synthetic fungicides. It is adapted to local environmental conditions, is easily and cheaply produced and can be stored in a low-cost formulation. Significance and Impact of the Study In Belgium, this is the first study to use aT. atroviridenative strain againstFusariumdamping-off on maize crop. Modes of action and required conditions for ensuring high biocontrol activity in the field have still to be investigated.