With the significant advances in electronic products, materials with good flexibility, corrosion resistance, high electrical conductivity and minimal thickness are urgently needed. Herein, we demonstrate the biomimetic core-shell structure of lightweight, flexible, self-cleaning nanofiber films for high-performance electromagnetic interference (EMI) shielding by tuning the deposition of Ag nanoparticles (AgNPs). With a thickness of 0.06 mm, PAN@TiO2@AgNPs composite films (PTA films) exhibit an average EMI shielding effectiveness (SE) of 82.60 dB. After further processing with fluorine-containing molecules, the PTA-4 film becomes superhydrophobic and anticorrosive. After a hydrophobic treatment, composite films have average SE, specific SE (SSE) and SSE/t being 79.57 dB, 360.86 dB cm(3) g(-1), and 60143.33 dB cm(2) g(-1), respectively. In particular, conductive films that undergo UV radiation and bending cycles retain a stabilized electrical conductivity. This tuning bio-inspired fabrication method provides the films with UV-resistance, superhydrophobicity and EMI SE that fit the practical applications of wearable and flexible sensors.