The sea buckthorn extract used for this study was obtained from dry sea buckthorn berries. The aim of this study was to synthesize by high shear homogenization method and to characterize new solid lipid nanoparticles loaded with sea buckthorn extract, nanoparticles which show high antioxidant activity. The obtained solid lipid nanoparticles have been firstly characterized by particle diameter and physical stability using dynamic light scattering method. The particle morphology was examined by transmission electron microscopy and the properties of sea buckthorn extract-loaded nanoparticles have been evaluated by an appropriate in vitro analysis (chemiluminescence method). The antimicrobial properties of newly developed nano-materials were also evaluated.