The increase of diesel vehicle leads to a large amount of NO emissions, and non-thermal plasma (NTP) combined with wood fiber is a new method in the field of exhaust gas purification. In this paper, the effects of NTP combined with different kinds of wood fibers and without wood fibers on the removal of NO were compared, and the optimal purification efficiency of NO was determined by experiments under different application conditions. The experimental results show that the NO conversion efficiency of NTP combined with wood fiber is better than that of NTP or wood fiber alone, among which NTP combined with scotch pine has the highest conversion efficiency. Through the analysis of the mechanism of NO conversion by wood and NTP, the effects of different conditions on NO conversion were obtained. The trend of NO removal with voltage/frequency indicates that the increase of voltage and frequency can promote the removal of NO. The increase of humidity also promoted the removal of NO, but when the humidity exceeded 25%, the conversion of NO decreased slightly. And the filling rate of 0.3 is the best value for NO conversion. Although NO cannot be completely purified by this method, there is still a higher conversion efficiency under the condition of low temperature and no catalyst. It can be proved that NTP bonded wood fiber is a new way to remove NO.