The next-generation bioresorbable polymeric stents are undergoing continual research and development, especially how to control and optimize their degradation behaviors. The aim of this paper is to study the properties of a poly(l-lactide) (PLLA) tube over accelerated degradation in vitro. Spherical nanoindentation was particularly adopted to measure the mechanical properties of PLLA tube; while differential scanning calorimetry and gel permeation chromatography were used for crystallinity and molecular weight measurements. Overall, the material degradation was captured by the local stress-strain response obtained with spherical nanoindentation, and further confirmed with chemical analyses. The results are particularly useful for development of bioresorbable polymeric stents with improved performance.