Microalgae have been recognized as a potential feedstock for biodiesel production due to their fast growth rate and ability to accumulate lipid within their cells. However, high lipid content and biomass productivity are usually difficult to achieve simultaneously. Instead of stressing the microalgae during cultivation stage, the stress environment such as nutrient starvation, salinity and light effect were introduced to Chlorella vulgaris after harvesting to study the effect on their lipid content. From the results attained, one day of nutrient starvation with 6.0 g/L of salinity stress under dark room condition had shown the highest lipid content of 38.8% (dry weight basis). The lipid content was recorded at 40.28% (dry weight basis) when the working volume was scaled up. In addition, the fatty acids identified in the extracted microalgae lipid were mainly consisted of linoleic, linolenic and palmitic acid, which were commonly found in corn oil, soybean oil and sunflower oil. (C) 2020 Elsevier Ltd. All rights reserved.