Preparation of a photocured GelMA hydrogel co-cultured with HOKs and HGFs for an artificial oral mucosal tissue model

So Yeon Kim; Yu-Shik Hwang; Heung Jae Chun; Dae Hyeok Yang

Journal of Industrial and Engineering Chemistry, Vol.89, 470-475, September, 2020

DOI10.1016/j.jiec.2020.06.025 Export Citation

Preparation of a photocured GelMA hydrogel co-cultured with HOKs and HGFs for an artificial oral mucosal tissue model

So Yeon Kim¹, Yu-Shik Hwang², Heung Jae Chun^{3, 4}, and Dae Hyeok Yang^{4, †}

¹Department of Dental Hygiene, College of Health Sciences, Cheongju University, Cheongju 28503, Republic of Korea
²Department of Maxillofacial Biomedical Engineering and Institute of Oral Biology, School of Dentistry, Kyung Hee University, Seoul 02447, Republic of Korea
³Department of Medical Life Sciences, College of Medicine, The Catholic University of Korea, Seoul 06591, Republic of Korea
⁴Institute of Cell and Tissue Engineering, College of Medicine, The Catholic University of Korea, Seoul 06591, Republic of Korea

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Reversible or irreversible damage causes oral mucosal defects, the treatment of which requires biomaterials with good biomechanical properties. In this study, we prepared an artificial oral mucosa tissue model using a photocured methacrylate gelatin (GelMA) hydrogel co-cultured with human gingival fibroblasts (HGFs) and human oral keratinocytes (HOKs). GelMA was fabricated via a chemical reaction between gelatin and methacrylic anhydride (MA), and its structure was confirmed via 1H NMR analysis. The culture of HOKs and HGFs was observed on the surface and in the bulk of the GelMA hydrogel, indicating that the hydrogel was biocompatible for cell adhesion and proliferation. In addition, immunofluorescent staining results indicated that HOKs form a three-dimensional cell structure while maintaining their intrinsic keratinocyte properties. Histological evaluations demonstrated that the HGF and HOK bi-layered GelMA hydrogel can be used to develop artificial oral mucosa tissue.

Keywords:Human gingival fibroblast;Human oral keratinocyte;Photocured methacrylate gelatin hydrogel;Artificial oral mucosa tissue model;Three-dimensional cell structure

[References]

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[Referenced By]

[1] Piemonte ED, Lazos JP, Brunotto M, J. Oral Pathol. Med., 39, 513 (2010)

[2] Zhang Q, Qing C, Xiao W, Zhu H, Guo J, Ge Z, Cui W, RSC Adv., 9, 18344 (2019)

[3] Yu J, Huang TR, Lim ZH, Luo R, Rasula RR, Liao LD, Lim S, Chen CH, Adv. Healthc. Mater., 5, 2983 (2016)

[4] Ponec M, Kempenaar J, Skin Pharm. Physiol., 8, 49 (1995)

[5] Ueda M, Hata K, Horie K, Torii S, Ann. Plast. Surg., 35, 498 (1995)

[6] Roh JL, Lee J, Kim EH, Shin D, Oral Oncol., 75, 81 (2017)

[7] Helary C, Bataille I, Abed A, Illoul C, Anglo A, Louedec L, Letourneur D, Meddahi-Pelle A, Giraud-Guille MM, Biomaterials, 31, 481 (2010)

[8] Van den Bulcke AI, Bogdanov B, De Rooze N, Schacht EH, Cornelissen M, Berghmans H, Biomacromolecules, 1(1), 31 (2000)

[9] Yang DH, Seo DI, Lee DW, Bhang SH, Park KS, Jang G, Kim CH, Chun HJ, J. Ind. Eng. Chem., 53, 360 (2017)

[10] Yoo Y, Yoon SJ, Kim SY, Lee DW, Um S, Hyun H, Hong SO, Yang DH, Drug Deliv., 25, 1664 (2018)

[11] Yoo SJ, Yoo Y, Nam SE, Hyun H, Lee DW, Um S, Kim SY, Hong SO, Yang DH, Chun HJ, Mar. Drugs, 16, 351 (2018)

[12] Bott K, Upton Z, Schrobback K, Ehrbar M, Hubbell JA, Lutolf MP, Rizzi SC, Biomaterials, 31, 8454 (2010)

[13] Lu G, Ling K, Zhao P, Xu Z, Deng C, Zheng H, Huang J, Chen J, Wound Repair Regen., 18, 70 (2010)

[14] Wu X, Liu Y, Liu A, Wang W, Int. J. Biol. Macromol., 98, 292 (2017)

[15] Zhu B, Li W, Chi N, Lewis RV, Osamor J, Wang R, ACS Omega, 2, 2439 (2017)

[16] Fu F, Chen Z, Zhao Z, Wang H, Shang L, Gu Z, Zhao Y, Proc. Natl. Acad. Sci., 114(1), 5900 (2017)

[17] Van den Bulcke AI, Bogdanov B, De Rooze N, Schacht EH, Cornelissen M, Berghmans H, Biomacromolecules, 1(1), 31 (2000)

[18] Grøn B, Stoltze K, Andersson A, Dabelsteen E, Apmis, 110, 892 (2002)

[19] Shirahama H, Lee BH, Tan LP, Cho NJ, Sci. Rep., 6, 1 (2016)

[20] Lee BH, Lum N, Seow LY, Lim PQ, Tan LP, Materials, 9, 797 (2016)

[21] Schuurman W, Levett PA, Pot MW, van Weeren PR, Dhert WJ, Hutmacher DW, Melchels FP, Klein TJ, Malda J, Macromol. Biosci., 13, 551 (2013)

[22] Klausner M, Ayehunie S, Breyfogle BA, Wertz PW, Bacca L, Kubilus J, Toxicol. Vitro, 21, 938 (2007)

[23] Zhao X, Liu S, Yildirimer L, Zhao H, Ding RH, Wang HN, Cui WG, Weitz D, Adv. Funct. Mater., 26(17), 2809 (2016)

[24] Oda D, Watson E, In Vitro Cell. Dev. Biol., 26, 589 (1990).

[25] Huynh NCN, Everts V, Leethanakul C, Pavasant P, Ampornaramveth RS, PLoS One, 11 (2016).

[26] Dongari-Bagtzoglou A, Kashleva H, Nat. Protoc., 1, 2012 (2006)

[27] Kim SY, Park BJ, Lee YK, Park NJ, Park KM, Hwang YS, Park KD, J. Ind. Eng. Chem., 73, 142 (2019)

[28] Degen M, Wiederkehr A, La Scala GC, Carmann C, Schnyder I, Katsaros C, Front. Physiol., 9, 1703 (2018)

[29] Jennings LR, Colley HE, Ong J, Panagakos F, Masters JG, Trivedi HM, Murdoch C, Whawell S, Tissue Eng. Part C Methods, 22, 1108 (2016)

[30] Lee HG, Eun HC, J. Dermatol. Sci., 21, 176 (1999)

[31] Porock D, Eur. J. Cancer Care, 11, 33 (2002)

[32] Turabelidze A, Guo S, Chung AY, Chen L, Dai Y, Marucha PT, DiPietro LA, PLoS One, 9 (2014).

[33] Ozaki T, Nishimura N, Muto T, Sugata K, Kawabe S, Goto K, Koyama K, Fujita H, Takahashi Y, Akiyama M, Vaccine, 23, 1205 (2005)

[34] Costantini M, Testa S, Fornetti E, Barbetta A, Trombetta M, Cannata SM, Gargioli C, Rainer A, Front. Bioeng. Biotechnol., 5, 22 (2017)

[35] McBeth C, Lauer J, Ottersbach M, Campbell J, Sharon A, Sauer-Budge AF, Biofabrication, 9, 015009 (2017)

[36] Zhao X, Sun X, Yildirimer L, Lang Q, Lin ZYW, Zheng R, Zhang Y, Cui W, Annabi N, Khademhosseini A, Acta biomater., 49, 66 (2017)

[37] Sun X, Lang Q, Zhang H, Cheng L, Zhang Y, Pan G, Zhao X, Yang H, Zhang Y, Santos HA, Adv. Funct. Mater., 27, 160461 (2017)

[38] Berndt A, Borsi L, Hyckel P, Kosmehl H, J. Cancer Res. Clin. Oncol., 127, 286 (2001)

[39] Coulomb B, Lebreton C, Dubertret L, J. Invest. Dermatol., 92, 122 (1989)

[40] Demarchez M, Hartmann DJ, Regnier M, Asselineau D, Transplantation, 54, 317 (1992)

[41] Bucchieri F, Fucarino A, Gammazza AM, Pitruzzella A, Marciano V, Paderni C, De Caro V, Siragusa MG, Muzio LL, Holgate ST, Curr. Pharm. Des., 18, 5421 (2012)

[42] Mitura S, Sionkowska A, Jaiswal A, J. Mater. Sci. -Mater. Med., 31, 1 (2020)