Comparison between Basic and Inverse Dual Drug and Peptide-coated Stents in a Porcine Restenosis Model

Eun-Jae Jang; So-Youn Lee; In-Ho Bae; Dae Sung Park; Myung Ho Jeong; Jun-Kyu Park

Applied Chemistry for Engineering, Vol.31, No.5, 502-508, October, 2020

DOI10.14478/ace.2020.1050 Export Citation

Comparison between Basic and Inverse Dual Drug and Peptide-coated Stents in a Porcine Restenosis Model

Eun-Jae Jang¹, So-Youn Lee^{2, 3}, In-Ho Bae^{2, 3}, Dae Sung Park^{2, 3}, Myung Ho Jeong^{2, 3, †}, and Jun-Kyu Park^{4, †}

¹Biopharmaceutical Research Center, Hwasun 58141, Korea
²The Cardiovascular Convergence Research Center of Chonnam National University, Hospital Designated by Ministry of Health and Welfare, Gwangju 61469, Korea
³Korea Cardiovascular Stent Research Institute, Jangsung 57248, Korea
⁴CGBio Co. Ltd, Seongnam 13211, Korea

E-mail:,

Dual drug-eluting stents (DES) is a primary treatment method for coronary arterial diseases in current interventional cardiology practice. However, their pathological results according to the sequence of coating of drugs have not been reported yet. The peptide-dopamine dissolved in acetonitrile was coated onto the Chonnam National University Hospital (CNUH) stent using an electrospinning coating machine. For secondary coating (e.g., sirolimus coating, designated as SPS), sirolimus (SRL) and poly lactic-glycolic acid (PLGA) were mixed in tetrahydrofuran (THF), and the solution was then coated on the CNUH stent that had underwent the primary peptide coating using an electrospinning and spray technique. Next, the peptide-dopamine was coated on the SRL-PLGA coated stent (PSS). In this study, it was confirmed that endothelialization was promoted without being significantly affected by the coating order (SPS or PSS). The sequence of drug and peptide coating may affect the development of restenosis and PSS was effective in the prevention of restenosis compared to that of using SPS.

Keywords:Dual drug eluting stent;Electro-spinning coating;Peptide;Endothelialization;Restenosis

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[2] Chang H, Ren KF, Zhang H, Wang jL, Wang BL, Ji J, J. Biomed. Mater. Res. B Appl. Biomater., 103, 430 (2015)

[3] Ma X, Oyamada S, Gao F, Wu T, Robich MP, Wu H, Wang X, et al., J. Pharm. Biomed. Anal., 54, 807 (2011)

[4] Huang Y, Venkatraman SS, Boey FY, Lahti EM, et al., Biomaterials, 31, 4382 (2010)

[5] Kivela A, Hartikainen J, Ann. Med., 38, 173 (2006)

[6] Lin Q, Ding X, Qiu F, Song X, Fu G, Ji J, Biomaterials, 31, 4017 (2010)

[7] Kleinedler JJ, Foley JD, Orchard EA, Dugas TR, J. Control. Release, 159, 27 (2012)

[8] Shuchman M, N. Engl. J. Med., 355, 1949 (2006)

[9] Tang H, Wanag Q, Wang X, Zhou J, Zhu M, Qiao T, Liu C, et al., J. Atheroscler. Thromb., 23, 67 (2016)

[10] Nakazawa G, Granada JF, Alviar CL, Tellez A, Kaluza GL, et al., JACC Cardiovasc. Interv., 3, 68 (2010)

[11] Bennett MR, Heart, 93, 895 (2007)

[12] Roopmani P, Satheesh S, Raj DC, Krishnan UM, ACS Biomater. Sci. Eng., 5(6), 2899 (2019)

[13] Yu M, Xu B, Kandzari DE, Wu Y, Yan H, Chen J, Qian J, Qiao S, Yang Y, Gao RL, Catheter Cardiovasc. Interv., 83, 405 (2014)

[14] Matter CM, Rozenberg I, Jaschko A, Greutert H, Kurz DJ, et al., J. Cardiovasc. Pharmacol., 48, 286 (2006)

[15] Curcio A, Torella D, Indolfi C, Circ. J., 75, 1287 (2011)

[16] Le Y, Gong W, Li B, Dunlop NM, Shen W, Su SB, Ye RD, Wang JM, J. Immunol., 163, 6777 (1999)

[17] Seo JK, Choi SY, Kim Y, Baek SH, Kim KT, Chae CB, Lambeth JD, Suh PG, Ryu SH, J. Immunol., 158, 1895 (1997)

[18] Heo SC, Kwon YW, Jang IH, Jeong GO, Yoon JW, Kim CD, Kwon SM, Bae YS, Kim JH, Stem Cells, 32, 779 (2014)

[19] Jang EJ, Bae IH, Park DS, Lee SY, Lim KS, Park JK, Shim JW, Sim DS, Jeong MH, J. Mater. Sci. -Mater. Med., 26, 251 (2015)

[20] Lee SY, Bae IH, Park DS, Jang EJ, Shim JW, Lim KS, Park JK, Sim DS, Jeong MH, J. Biomed. Mater. Res. A, 105, 301 (2017)

[21] Bae IH, Lim KS, Park JK, Park DS, Lee SY, Jang EJ, Ji MS, Sim DS, Hong YJ, Ahn Y, Park JC, Cho JG, Kang JC, Kim IS, Nah JW, Jeong MH, J. Ind. Eng. Chem., 21, 1295 (2015)

[22] Buszman P, Trznadel S, Zurakowski A, Milewski K, Kinasz L, Krol M, Kondys M, Kardiol. Pol., 65, 1041 (2007)

[23] National Research Council (U.S.), Guide for the care and use of laboratory animals, National Academies Press,, Washington, D.C., pp. xxv, p. 220 2011.

[24] Schwartz RS, Huber KC, Murphy JG, Edwards WD, Camrud AR, Vlietstra RE, Holmes DR, J. Am. Coll. Cardiol., 19, 267 (1992)

[25] Chen MH, Liang PC, Chang KC, Huang JY, Chang YT, Chang FY, Wong JM, Lin FH, Biomed. Eng. Online, 13, 118 (214)

[26] Ye W, Shi Q, Hou JW, Jin J, Fan QF, Wong SC, Xu XD, Yin JH, J. Mater. Chem. B, 2, 7186 (2014)

[27] Heldman AW, Cheng L, Jenkins GM, Heller PF, et al., Circulation, 103, 2289 (2001)

[28] Choi YH, Heo SC, Kwon YW, Kim HD, Kim SH, Jang IH, Kim JH, Hwang NS, Acta Biomater., 25, 76 (2015)