Evaluation of Hyaluronic Acid/Agarose Hydrogel for Cartilage Tissue Engineering Biomaterial

Joo Hee Choi; Jin Su Kim; Won Kyung Kim; Wonchan Lee; Namyeong Kim; Cheol Ui Song; Jun Jae Jung; Jeong Eun Song; Gilson Khang

Macromolecular Research, Vol.28, No.11, 979-985, October, 2020

DOI10.1007/s13233-020-8137-6 Export Citation

Evaluation of Hyaluronic Acid/Agarose Hydrogel for Cartilage Tissue Engineering Biomaterial

Joo Hee Choi, Jin Su Kim, Won Kyung Kim, Wonchan Lee, Namyeong Kim, Cheol Ui Song, Jun Jae Jung, Jeong Eun Song, and Gilson Khang^†

Department of BIN Convergence Technology, Department of Polymer Nano Science & Technology and Polymer BIN Research Center, Jeonbuk National University, Deokjin-gu, Jeonju-si, Jeonbuk 54896, Korea

E-mail:

Hyaluronic acid (HA) is one of the most applied biomaterials in a tissue engineering field due to its biocompatibility and its presence in the native extracellular matrix (ECM) of tissues. However, the mechanical property of the HA is weak and requires specific treatment to improve its properties. The application of Agarose (AG) hydrogel is widely studied and used as a support for the three-dimensional culture of cells due to its biocompatibility. Nevertheless, AG itself lacks the biological environment of the matrix which is unsuitable for the growth of the encapsulated cells. In this study, the composite of HA hydrogel and AG hydrogel (HA/AG hydrogel) is proposed to supplement the drawbacks of each hydrogel. HA provided enhanced microenvironment of matrix and AG improved the mechanical properties and assisted the cells. The characterization of the blended hydrogels was carried out with FT-IR, weight loss, swelling ratio, and compressive strength study. The biocompatibility and biological environment of the composite was evaluated by dimethylthiazol-2-yl-2,5-diphenyltetrazolium bromide; thiazolyl blue (MTT), live/dead staining, and morphological study. The composite biomaterial exhibited applicability for cartilage tissue engineering and the in vitro study of the cell-laden HA/AG hydrogel displayed potential for cartilage tissue engineering in the future.

Keywords:agarose;hyaluronic acid;hydrogel;cartilage;regeneration

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[2] Ren KX, He CL, Xiao CS. Li G, Chen XS, Biomaterials, 51, 238 (2015)

[3] Lees D, Partington P, Orthop. Trauma, 30, 265 (2016)

[4] Zhang KX, Yan SF, Li GF, Cui L, Yin JB, Biomaterials, 71, 24 (2015)

[5] Fischer S, Kisser A, J. Orthop., 13, 246 (2016)

[6] Yasui Y, Ando W, Shimomura K, Koizumi K, Ryota C, Hamamoto S, Kobayashi M, Yoshikawa H, Nakamura N, J. Clin. Orthop. Trauma, 7, 157 (2016)

[7] Zhang XL, Wu Y, Pan ZY, Sun H, Wang JJ, Yu DS, Zhu S, Dia Y, et al., Acta Biomater., 42, 329 (2016)

[8] Zhang YY, Yang F, Liu K, Shen H, Zhu YD, Zhang WJ, Liu W, Wang SG, Cao YL, Zhou GD, Biomaterials, 33, 2926 (2012)

[9] Mumme M, Barbero A, Miot S, Wixmerten A, Feliciano S, Wolf F, et al., Lancet, 388, 1985 (2016)

[10] Kim IG, Ko j, Lee HR, Do SH, Park K, Biomaterials, 85, 18 (2016)

[11] Kuo YC, Wang CC, Colloids Surf. B: Biointerfaces, 93, 235 (2012)

[12] Hawkins AM, Milbrandt TA, Puleo DA, Hilt JZ, Acta Biomater., 7, 1956 (2011)

[13] Ahn HH, Kim KS, Lee jH, Lee JY, Kim BS, Lee IW, Chun HJ, Kim IH, Lee HB, Kim MS, Tissue Eng. Part A, 15, 1821 (2009)

[14] Yang DH, Kim HJ, Kim JK, Chun HJ, Park K, J. Ind. Eng. Chem., 45, 156 (2017)

[15] Deshmukh M, Singh Y, Gunaseelan S, Gao DY, Stein S, Sinko PJ, Biomaterials, 31, 6675 (2010)

[16] Ono S, Ogawa R, Hyakusoku H, Plast. Reconstr. Surg., 126, 1349 (2010)

[17] Qiao Q, Wang X, Sun J, Zhao R, Liu Z, Wang Y, Sun B, Yan Y, Qi K, Aesthetic Plast. Surg., 29, 156 (2005)

[18] Toole BP, Nat. Rev. Cancer, 4, 528 (2004)

[19] Tan H, Chu CR, Payne KA, Marra KG, Biomaterials, 30, 2499 (2009)

[20] Park H, Choi B, Hu J, Lee M, Acta Biomater., 9, 4779 (2013)

[21] Spiller KL, Maher SA, Lowman AM, Tissue Eng. Part B: Rev., 17, 281 (2011)

[22] Kim IL, Mauck RL, Burdick JA, Biomaterials, 32, 8771 (2011)

[23] Segura T, Anderson BC, Chung PH, Webber RE, Shull KR, Shea LD, Biomaterials, 26, 359 (2005)

[24] Zeng Q, Han Y, Li H, Chang J, J. Mater. Chem. B, 3, 8856 (2015)

[25] Tripathi A, Kathuria N, Kumar A, J. Biomed. Mater. Res. A, 90, 680 (2009)

[26] Gros T, Sakamoto JS, Blesch A, Havton LA, Tuszynski MH, Biomaterials, 31, 6719 (2010)

[27] Zhang LM, Wu CX, Huang JY, Peng XH, Chen P, Tang SQ, Carbohydr. Polym., 88, 1445 (2012)

[28] Lang A, Thorpe M, J. Experim. Botany, 40, 1069 (1989)

[29] Karageorgiou V, Kaplan D, Biomaterials, 26, 5474 (2005)

[30] Liu J, Zheng X, Tang K, Rev. Adv. Mater. Sci., 33, 452 (2013)

[31] Hutmacher DW, Biomaterials, 21, 175 (2000)

[32] Bose S, Roy M, Bandyopadhyay A, Trends Biotechnol., 30, 546 (2012)

[33] Nava MM, Draghi L, Giordano C, Pietrabissa R, J. Appl. Biomater. Funct. Mater., 14, e223 (2016)

[34] Berthod F, Saintigny G, Chretien F, Hayek D, Collombel C, Damour O, Clin. Mater., 15, 259 (1994)

[35] Peppas NA, Hilt JZ, Khademhosseini A, Langer R, Adv. Mater., 18(11), 1345 (2006)

[36] Khademhosseini A, Langer R, Biomaterials, 28, 5087 (2007)

[37] Nichol JW, Khademhosseini A, Soft Matter, 5, 1312 (2009)

[38] Annabi N, Nichol JW, Zhong X, Ji C, Koshy S, Khademhosseini A, Dehghani F, Tissue Eng. Part B: Rev., 16, 371 (2010)

[39] Liao J, Qu Y, Chu B, Zhang X, Qian Z, Sci. Rep., 5, 9879 (2015)

[40] Tonda-Turo C, Gnavi S, Ruini F, Gambarotta G, Gioffredi E, Chiono V, Perroteau I, Ciardelli G, J. Tissue. Eng. Regen. Med., 11, 197 (2017)

[41] Kamath KR, Park K, Adv. Drug Deliv. Rev., 11, 59 (1993)

[42] Bryant SJ, Bender RJ, Durand KL, Anseth KS, Biotechnol. Bioeng., 86(7), 747 (2004)

[43] Ballios BG, Cooke MJ, van der Kooy D, Shoichet MS, Biomaterials, 31, 2555 (2010)

[44] Kim WK, Choi JH, Shin ME, Kim JW, Kim PY, Kim N, Song JE, Khang G, Int. J. Biol. Macromol., 141, 51 (2019)

[45] Necas J, Bartosikova L, Brauner P, Kolar J, Vet. Med., 53, 397 (2008)

[46] Aruffo A, Stamenkovic I, Melnick M, Underhill CB, Seed B, Cell, 61, 1303 (1990)

[47] Wang K, Bruce A, Mezan R, Kadiyala A, Wang L, Dawson J, Rojanasakul Y, Yang Y, ACS Appl. Mater. Interfaces, 8, 5082 (2016)

[48] Kalantary S, Jahani A, Pourbabaki R, Beigzadeh Z, RSC Adv., 9, 24858 (2019)

[49] Kalantary S, Golbabaei F, Latifi M, Shokrgozar MA, Yaseri M, SN Appl. Sci., 1, 1042 (2019)

[50] Kang YM, Lee SH, Lee JY, Son JS, et al., Biomaterials, 31, 2453 (2010)

[51] Kalantary S, Golbabaei F, Latifi M, Shokrgozar MA, Yaseri M, J. Nanosci. Nanotechnol., 20, 3554 (2020)