화학공학소재연구정보센터
홈 로그인 로그아웃 연락처 사이트맵
Journal of Industrial and Engineering Chemistry, Vol.91, 37-53, November, 2020
DOI10.1016/j.jiec.2020.08.009  Export Citation
Cell death in culture: Molecular mechanisms, detections, and inhibition strategies
Anandrao Ashok Patil1, 2, Sachin Ashok Bhor3, and Won Jong Rhee1, 4, †
  • 1Division of Bioengineering, Incheon National University, 119 Academy-ro, Yeonsu-gu, Incheon 22012, Republic of Korea
  • 2Department of Entomology and Plant Pathology, CALS, North Carolina State University, Raleigh, NC 27615, USA
  • 3The United Graduate School of Agricultural Sciences, Ehime University, Matsuyama, Ehime 790-8566, Japan
  • 4Department of Bioengineering and Nano-Bioengineering, Incheon National University, 119 Academy-ro, Yeonsu-gu, Incheon 22012, Republic of Korea
E-mail:
Mammalian cell cultures are widely used in the biopharmaceutical industry to produce monoclonal antibodies, vaccines, growth factors, etc. Cell death is an essential biological process for physiological growth and development, but it is a major problem in biopharmaceutical production in bio-industry. Cell death within bioreactor occurs due to various intracellular and extracellular stresses. These stresses negatively affect the culture longevity, overall product quality, and yield. Among all cell death types, apoptosis accounts for most of the cellular death in the bioreactor. The implementation and development of various strategies to prevent the cellular death are crucial for robust bioprocess development. Cell death during culture can be prevented or inhibited by supplementing media with specific chemicals, synthetic inhibitors, and genetic cell engineering approaches. In this review, we classified and described different types of cell death and their molecular mechanisms and summarized the cell death inhibition approaches implemented to inhibit cell death for various applications.
Keywords:Cell death;Apoptosis;Anti-apoptosis;Cell engineering;Cell death inhibition
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