Tyrosinase-mediated rapid and permanent chitosan/gelatin and chitosan/gelatin/nanohydroxyapatite hydrogel

Sujin Lim; Dawoon Jeong; Mi-Ran Ki; Seung Pil Pack; Yoo Seong Choi

Korean Journal of Chemical Engineering, Vol.38, No.1, 98-103, January, 2021

DOI10.1007/s11814-020-0672-5 Export Citation

Tyrosinase-mediated rapid and permanent chitosan/gelatin and chitosan/gelatin/nanohydroxyapatite hydrogel

Sujin Lim, Dawoon Jeong, Mi-Ran Ki¹, Seung Pil Pack¹, and Yoo Seong Choi^†

Department of Chemical Engineering and Applied Chemistry, Chungnam National University, Daejeon 34134, Korea
¹Department of Biotechnology and Bioinformatics, Korea University, Sejong 30019, Korea

E-mail:

Chitosan/gelatin and chitosan/gelatin/nanohydroxyapatite hydrogels were rapidly and stably prepared without any crosslinking materials by using an engineered tyrosinase (mTyr-CNK) with high catalytic activity for tyrosine/ DOPA-tethered polymeric biomaterials throughout a broad pH range. A dual-barrel syringe with one part containing chitosan/mTyr-CNK solution and the other containing gelatin solution with/without nanohydroxyapatite was successfully used to form homogeneous hydrogels at room temperature followed by 37 °C to simulate an in situ injection approach. The obtained hydrogels exhibited an average pore size greater than 150 μm and high swelling ratios with similar mechanical properties to other chemically crosslinked chitosan/gelatin hydrogels. The in vitro degradation properties and cellular viability suggested that the hydrogels could be used as biodegradable and biocompatible scaffolds for biomedical applications, such as space filling biomaterials and delivery vehicles for bioactive molecules and cells. These results demonstrated that mTyr-CNK-mediated hydrogels have remarkable promise as an injectable scaffold biomaterial.

Keywords:Chitosan;Gelatin;Nanohydroxyapatite;Hydrogel;Tyrosinase

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[2] Liu M, Zeng X, Ma C, Yi H, Ali Z, Mou XB, Li S, Deng Y, He NY, Bone Res., 5, 17014 (2017)

[3] Patterson J, Martino MM, Hubbell JA, Mater. Today, 13, 14 (2010)

[4] Li YL, Rodrigues J, Tomas H, Chem. Soc. Rev., 41, 2193 (2012)

[5] LogithKumar R, KeshavNarayan A, Dhivya S, Chawla A, Saravanan S, Selvamurugan N, Carbohydr. Polym., 151, 172 (2016)

[6] Zhu LD, Bratlie KM, Biochem. Eng. J., 132, 38 (2018)

[7] Su K, Wang CM, Biotechnol. Lett., 37(11), 2139 (2015)

[8] Yang YJ, Choi YS, Cha HJ, Biotechnol. J., 13, 180008 (2018)

[9] Cui L, Jia JF, Guo Y, Liu Y, Zhu P, Carbohydr. Polym., 99, 31 (2014)

[10] Saraiva SM, Miguel SP, Ribeiro MP, Coutinho P, Correia IJ, RSC Adv., 5, 63478 (2015)

[11] Nieto-Suarez M, Lopez-Quintela MA, Lazzari M, Carbohydr. Polym., 141, 175 (2016)

[12] Choi YR, Kim EH, Lim S, Choi YS, Biochem. Eng. J., 129, 50 (2018)

[13] Bai X, Gao M, Syed S, Zhuang J, Xu X, Zhang XQ, Bioact. Mater., 3, 401 (2018)

[14] Otsuka M, Biol. Pharm. Bull., 36, 1653 (2013)

[15] Ohtsuki C, Kamitakahara M, Miyazaki T, J. R. Soc. Interface., 6, S349 (2009)

[16] Zhou H, Lee J, Acta Biomater., 7, 2769 (2011)

[17] LeGeros RZ, Clin. Mater., 14, 65 (1993)

[18] Stupp SI, Ciegler GW, J. Biomed. Mater. Res., 26, 169 (1992)

[19] Do H, Kang E, Yang B, Cha HJ, Choi YS, Sci. Rep., 7, 17267 (2017)

[20] Hafidz RNRM, Yaakob CM, Amin I, Noorfaizan A, Int. Food Res. J., 18, 787 (2011)

[21] Kim EH, Lim S, Kim TE, Jeon IO, Choi YS, Biotechnol. Bioprocess Eng., 23, 500 (2018)

[22] Yan CQ, Pochan DJ, Chem. Soc. Rev., 39, 3528 (2010)

[23] Guo L, Colby RH, Lusignan CP, Howe AM, Macromolecules, 36(26), 10009 (2003)

[24] Yan SF, Wang TT, Li X, Jian YH, Zhang KX, Li GF, Yin JB, RSC Adv., 7, 17005 (2017)

[25] Gariboldi MI, Best SM, Front Bioeng. Biotechnol., 3, 151 (2015)

[26] Anseth KS, Bowman CN, Brannon-Peppas L, Biomaterials, 17, 1647 (1996)

[27] Jiankang H, Dichen L, Yaxiong L, Bo Y, Hanxiang Z, Qin L, Bingheng L, Yi L, Acta Biomater., 5, 453 (2009)

[28] Pei YY, Guo DM, An QD, Xiao ZY, Zhai SR, Zhai B, Korean J. Chem. Eng., 35(12), 2384 (2018)

[29] Jo S, Park S, Oh Y, Hong J, Kim HJ, Kim KJ, Oh KK, Lee SH, Biotechnol. Bioprocess. Eng., 24, 145 (2019)