Correlation between fixation of high-concentration CO2 and glutamate accumulation in Sulfurovum lithotrophicum 42BKTT

Sewon Park; Hyuk-Sung Kwon; Chang-Ha Lee; Ik-Sung Ahn

Journal of Industrial and Engineering Chemistry, Vol.92, 56-61, December, 2020

DOI10.1016/j.jiec.2020.08.015 Export Citation

Correlation between fixation of high-concentration CO2 and glutamate accumulation in Sulfurovum lithotrophicum 42BKTT

Sewon Park, Hyuk-Sung Kwon¹, Chang-Ha Lee , and Ik-Sung Ahn^†

Department of Chemical and Biomolecular Engineering, Yonsei University, 50 Yonsei-ro, Seodaemun-gu, Seoul 03722, Republic of Korea
¹Technical Qualification Question-Making Bureau, Human Resources Development Services of Korea, 345 Jongga-ro, Jung-gu, Ulsan, Republic of Korea

E-mail:

Glutamate was identified as a compatible solute for Sulfurovum lithotrophicum 42BKTT, which is a marine chemolithoautotrophic bacterium and performs CO2 fixation through the reductive tricarboxylic acid (TCA) cycle. In a medium of 20 g/L or higher concentration of NaCl, sufficient to induce osmotic stress, the intracellular level of glutamate rapidly increased on exposure to high levels of NaHCO3. Correlation of the glutamate accumulation with the level of NaHCO3 was determined and compared to that with the level of NaCl. Glutamate synthesis driven by NaHCO3 was measured at 1.29 mg Glu/mg protein (Osmol/L), and 18.4 times higher than that driven by NaCl (0.07 mg Glu/mg protein/(Osmol/L)). Thus, the synthesis of glutamate from 2-oxoglutarate, which is the first product of CO2 assimilation in the reductive TCA cycle, was triggered at the exposure to the high level of NaHCO3 and appeared to support S. lithotrophicum 42BKTT via resistance to osmotic stress and CO2 toxicity.

Keywords:Chemolithaoutotroph;CO2 fixation;Compatible solute;Glutamate CO2 toxicity

[References]

Wennersten R, Sun Q, Li H, J. Clean Prod., 103, 724 (2015)
Rahman FA, Aziz MMA, Saidur R, Bakar WAWA, Hainin M, Putrajaya R, Hassan NA, Renew. Sust. Energ. Rev., 71, 112 (2017)
da Rosa GM, Moraes L, Cardias BB, de Souza MDAZ, Costa JAV, Bioresour. Technol., 192, 321 (2015)
Peng Y, Zhao B, Li L, Energy Procedia, 14, 1515 (2012)
Khatiwala S, Primeau F, Hall T, Nature, 462, 346 (2009)
Ho SH, Chen CY, Lee DJ, Chang JS, Biotechnol. Adv., 29, 189 (2011)
Kumar A, Ergas S, Yuan X, Sahu A, Zhang Q, Dewulf J, Malcata FX, Van Langenhove H, Trends Biotechnol., 28, 371 (2010)
Madigan MT, Martinko JM, Brock Biology of Microorganisms, (2014).
Kurosawa H, Nakagomi T, Kanda K, Nakamura K, Amano Y, J. Ferment. Bioeng., 72, 36 (1991)
Ramanan R, Kannan K, Deshkar A, Yadav R, Chakrabarti T, Bioresour. Technol., 101(8), 2616 (2010)
Yue LH, Chen WG, Energy Conv. Manag., 46(11-12), 1868 (2005)
Sung KD, Lee JS, Shin CS, Park SC, Renew. Energy, 16(1), 1019 (1999)
Silva HJ, Pirt SJ, Microbiology, 130, 2833 (1984)
Pirt MW, Pirt SJ, Microbiology, 119, 321 (1980)
Kwon HS, Lee JH, KimT, Kim JJ, JEon P, Lee CH, Ahn IS, RSC Adv., 5, 7151 (2015)
Inagaki F, Takai K, Nealson KH, Horikoshi K, Int. J. Syst. Evol. Microbiol., 54, 1477 (2004)
Kumar A, Bachhawat AK, Curr. Sci., 102, 288 (2012)
Botsford JL, Arch. Microbiol., 137, 124 (1984)
Measures J, Nature, 257, 398 (1975)
Tempest D, Meers J, Brown C, Microbiology, 64, 171 (1970)
Brown A, Bacteriol. Rev., 40, 803 (1976)
Makemson J, Hastings J, Appl. Environ. Microbiol., 38, 178 (1979)
Wood JM, Bremer E, Csonka LN, Kraemer R, Poolman B, van der Heide T, Smith LT, Comp. Biochem. Physiol. Part A: Mol. Integr. Physiol., 130, 437 (2001).
Yancey PH, Clark ME, Hand SC, Bowlus RD, Somero GN, Science, 217, 1214 (1982)
Csonka LN, Microbiol. Rev., 53, 121 (1989)
Houghton R, Annu. Rev. Earth Planet. Sci., 35, 313 (2007)
Jeon W, Priscilla L, Park G, Lee H, Lee N, Lee D, Kwon H, Ahn I, Lee C, Lee H, Stand. Genomic Sci., 12, 54 (2017)
Kwon HS, Park SW, Lee CH, Ahn IS, J. Ind. Eng. Chem., 57, 72 (2018)
Bhargava S, Kachouli R, Maithil R, Kaithwas V, Microbiology, 80, 461 (2011)
Smart KF, Aggio RB, Van Houtte JR, Villas-Boas SG, Nat. Protoc., 5, 1709 (2010)
Bradford MM, Anal. Biochem., 72, 248 (1976)
Rasouli M, Clin. Biochem., 49, 936 (2016)
Sarbar M, Covington A, Nuttall R, Goldberg R, J. Chem. Thermodyn., 14, 967 (1982)
Robinson RA, Stokes RH, Trans. Faraday Soc., 45, 612 (1949)
Freedman DA, Statistical Models: Theory and Practice, Cambridge University Press, 2009.
Chernov N, Circular and Linear Regression: Fitting Circles and Lines by Least Squares, CRC Press, 2010.
Grabowska A, Nowicki M, Kwinta J, Acta Physiol. Plant., 33, 1981 (2011)
Temple SJ, Vance CP, Gantt JS, Trends Plant Sci., 3, 51 (1998)

[Related Articles]

[1] Wennersten R, Sun Q, Li H, J. Clean Prod., 103, 724 (2015)

[2] Rahman FA, Aziz MMA, Saidur R, Bakar WAWA, Hainin M, Putrajaya R, Hassan NA, Renew. Sust. Energ. Rev., 71, 112 (2017)

[3] da Rosa GM, Moraes L, Cardias BB, de Souza MDAZ, Costa JAV, Bioresour. Technol., 192, 321 (2015)

[4] Peng Y, Zhao B, Li L, Energy Procedia, 14, 1515 (2012)

[5] Khatiwala S, Primeau F, Hall T, Nature, 462, 346 (2009)

[6] Ho SH, Chen CY, Lee DJ, Chang JS, Biotechnol. Adv., 29, 189 (2011)

[7] Kumar A, Ergas S, Yuan X, Sahu A, Zhang Q, Dewulf J, Malcata FX, Van Langenhove H, Trends Biotechnol., 28, 371 (2010)

[8] Madigan MT, Martinko JM, Brock Biology of Microorganisms, (2014).

[9] Kurosawa H, Nakagomi T, Kanda K, Nakamura K, Amano Y, J. Ferment. Bioeng., 72, 36 (1991)

[10] Ramanan R, Kannan K, Deshkar A, Yadav R, Chakrabarti T, Bioresour. Technol., 101(8), 2616 (2010)

[11] Yue LH, Chen WG, Energy Conv. Manag., 46(11-12), 1868 (2005)

[12] Sung KD, Lee JS, Shin CS, Park SC, Renew. Energy, 16(1), 1019 (1999)

[13] Silva HJ, Pirt SJ, Microbiology, 130, 2833 (1984)

[14] Pirt MW, Pirt SJ, Microbiology, 119, 321 (1980)

[15] Kwon HS, Lee JH, KimT, Kim JJ, JEon P, Lee CH, Ahn IS, RSC Adv., 5, 7151 (2015)

[16] Inagaki F, Takai K, Nealson KH, Horikoshi K, Int. J. Syst. Evol. Microbiol., 54, 1477 (2004)

[17] Kumar A, Bachhawat AK, Curr. Sci., 102, 288 (2012)

[18] Botsford JL, Arch. Microbiol., 137, 124 (1984)

[19] Measures J, Nature, 257, 398 (1975)

[20] Tempest D, Meers J, Brown C, Microbiology, 64, 171 (1970)

[21] Brown A, Bacteriol. Rev., 40, 803 (1976)

[22] Makemson J, Hastings J, Appl. Environ. Microbiol., 38, 178 (1979)

[23] Wood JM, Bremer E, Csonka LN, Kraemer R, Poolman B, van der Heide T, Smith LT, Comp. Biochem. Physiol. Part A: Mol. Integr. Physiol., 130, 437 (2001).

[24] Yancey PH, Clark ME, Hand SC, Bowlus RD, Somero GN, Science, 217, 1214 (1982)

[25] Csonka LN, Microbiol. Rev., 53, 121 (1989)

[26] Houghton R, Annu. Rev. Earth Planet. Sci., 35, 313 (2007)

[27] Jeon W, Priscilla L, Park G, Lee H, Lee N, Lee D, Kwon H, Ahn I, Lee C, Lee H, Stand. Genomic Sci., 12, 54 (2017)

[28] Kwon HS, Park SW, Lee CH, Ahn IS, J. Ind. Eng. Chem., 57, 72 (2018)

[29] Bhargava S, Kachouli R, Maithil R, Kaithwas V, Microbiology, 80, 461 (2011)

[30] Smart KF, Aggio RB, Van Houtte JR, Villas-Boas SG, Nat. Protoc., 5, 1709 (2010)

[31] Bradford MM, Anal. Biochem., 72, 248 (1976)

[32] Rasouli M, Clin. Biochem., 49, 936 (2016)

[33] Sarbar M, Covington A, Nuttall R, Goldberg R, J. Chem. Thermodyn., 14, 967 (1982)

[34] Robinson RA, Stokes RH, Trans. Faraday Soc., 45, 612 (1949)

[35] Freedman DA, Statistical Models: Theory and Practice, Cambridge University Press, 2009.

[36] Chernov N, Circular and Linear Regression: Fitting Circles and Lines by Least Squares, CRC Press, 2010.

[37] Grabowska A, Nowicki M, Kwinta J, Acta Physiol. Plant., 33, 1981 (2011)

[38] Temple SJ, Vance CP, Gantt JS, Trends Plant Sci., 3, 51 (1998)