화학공학소재연구정보센터
홈 로그인 로그아웃 연락처 사이트맵
Journal of Industrial and Engineering Chemistry, Vol.94, 282-291, February, 2021
DOI10.1016/j.jiec.2020.11.001  Export Citation
Characterization and intracellular mechanism of electrospun poly (ε-caprolactone) (PCL) fibers incorporated with bone-dECM powder as a potential membrane for guided bone regeneration
Eunjeong Choi, Seungkuk Bae1, Dongyun Kim2, Gi Hoon Yang, KyoungHo Lee1, Hi-Jin You3, Hyo Jin Kang4, So-Jung Gwak5, SangHyun An1, †, and Hojun Jeon†
  • Research Institute of Additive Manufacturing and Regenerative Medicine, Baobab Healthcare Inc., 55 Hanyangdaehak-Ro, Ansan, Gyeonggi-Do 15588, South Korea
  • 1Laboratory Animal Center, Daegu-Gyeongbuk Medical Innovation Foundation (DGMIF), 80 Cheombok-ro, Dong-gu, Daegu 41061, South Korea
  • 2Department of Biomechatronic Engineering, College of Biotechnology and Bioengineering, Sungkyunkwan University, 2066 Seobu-ro, Suwon, Gyeonggi-Do 16419, South Korea
  • 3Department of Plastic Surgery, Korea University Ansan Hospital, 123 Jeokgeum-ro, Danwon-gu, Ansan-si, Gyeonggi-Do 15355, South Korea
  • 4Biomedical Research Center, Korea University Ansan Hospital, 123 Jeokgeum-ro, Danwon-gu, Ansan-si, Gyeonggi-Do 15355, South Korea
  • 5Department of Chemical Engineering, Wonkwang University, 460 Iksandae-ro, Iksan, Jeonbuk 54538, South Korea
E-mail:,
Various approaches have been made to successfully regenerate tissues or organs. One approach is to design a bioengineered scaffold which simulate the native environment of tissues and organs. This study examined the efficacy of a PCL based fibrous scaffold embedded with decellularized bone extracellular matrix (bdECM) fabricated using electrospinning process. Electrospun fibers are known to mimic the structure of the natural ECM, while bdECM can provide a rich environment for the human mesenchymal stem cells to adhere and differentiate. We found that the bdECM particles strongly influenced the surface characteristics of the fabricated scaffolds such as wettability, water uptake ability, and surface roughness. Also, the cell adhesion depending on the bdECM content was studied in depth. As a result, increased bdECM content in the scaffold showed enhanced cell adhesion and proliferation. Moreover, the ALP activity, calcium deposition, and gene expression results indicate that the bdECM particles had significant effect on osteogenic differentiation. In the present study, the feasibility of the bdECM/PCL scaffold was demonstrated which can be used as a potential biomedical device for hard tissue regeneration.
Keywords:Decellularized bone extracellular matrix;(bdECM);Electrospinning;Cell adhesion;Human mesenchymal stem cells;Osteogenic differentiation
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