Preparation of alginate hydrogel with human-derived adipose tissue to improve fat graft survival and adipogenesis

ByoungYong Yoo; Sujin Kim; Byung Ho Shin; Moon Hyun Lee; Young Bin Choy; Kangwon Lee; Chan Yeong Heo; Won-Gun Koh

Journal of Industrial and Engineering Chemistry, Vol.95, 148-155, March, 2021

DOI10.1016/j.jiec.2020.12.015 Export Citation

Preparation of alginate hydrogel with human-derived adipose tissue to improve fat graft survival and adipogenesis

ByoungYong Yoo¹, Sujin Kim², Byung Ho Shin³, Moon Hyun Lee⁴, Young Bin Choy^{5, 3}, Kangwon Lee^{6, †}, Chan Yeong Heo^{7, 8, †}, and Won-Gun Koh^{1, †}

¹Department of Chemical and Biomolecular Engineering, Yonsei University, Seoul, 03722, Republic of Korea
²Program in Nanoscience and Technology, Graduate School of Convergence Science and Technology, Seoul National University, Seoul, 08826, Republic of Korea
³Department of Biomedical Engineering, Seoul National University College of Medicine, Seoul 03080, Republic of Korea
⁴Nano Biomaterial Laboratory, Department of Polymer Science & Engineering, Sungkyunkwan University, Suwon, Republic of Korea
⁵Interdisciplinary Program for Bioengineering, College of Engineering, Seoul National University, Seoul 08826, Republic of Korea
⁶Department of Applied Bioengineering, Graduate School of Convergence Science and Technology, Seoul National University, Seoul, 08826, Republic of Korea
⁷Department of Plastic and Reconstructive Surgery, Seoul National University, College of Medicine, Seoul 03080, Republic of Korea
⁸Department of Plastic and Reconstructive Surgery, Seoul National University Bundang Hospital, Seongnam 13620, Republic of Korea

E-mail:, ,

Autologous fat and synthetic and natural polymers have been used as materials and scaffolds in adipose tissue (AD) regeneration, combining various polymers and biological components, including extracellular matrix molecules, decellularized matrix, and adipose-derived stem cells, which have been studied to improve the adipogenesis ability of scaffolds. This study used human AD as the scaffold material, with a great source of biomaterials closest to the human body. We report a simple and facile scaffold fabrication method using a natural polymer, alginate, and human AD and confirmed the adipogenesis ability in vivo. Scaffold fabrication was performed by simply mixing human AD with alginate and cross-linking in a non-cytotoxic way, using CaCl2 solution. This method allows facile control of the shape and mechanical properties of the scaffold, which has great advantages in medical and aesthetic applications. In vitro experiments demonstrated the viability and secretion of adipokines in the scaffold. Four weeks of in vivo experiments showed that alginate protected human AD, which aided in AD survival, and that volume and shape were better preserved. Furthermore, we confirmed that our scaffold enhanced the adipose regeneration of the host animal and increased adipogenesis of the transplanted human AD by gene expression analysis.

Keywords:Human adipose tissue;Alginate-fat scaffold;Adipose tissue regeneration

[References]

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[Referenced By]

[1] Alhadlaq A, Tang M, Mao JJ, Tissue Eng., 11, 556 (2005)

[2] Casadei A, Epis R, Ferroni L, Tocco I, Gardin C, Bressan E, Sivolella S, Vindigni V, Pinton P, Mucci G, Biomed Res. Int., 2012 (2012)

[3] Kokai LE, Marra K, Rubin JP, Transl. Res., 163, 399 (2014)

[4] Illouz YG, Sterodimas A, Aesthetic Plast. Surg., 33, 706 (2009)

[5] Cherubino M, Marra KG, Regen. Med. (2009).

[6] Arkudas A, Beier JP, Heidner K, Tjiawi J, Polykandriotis E, Srour S, Sturzl M, Horch RE, Kneser U, Tissue Eng., 13, 1549 (2007)

[7] Levy M, Luciani N, Alloyeau D, Elgrabli D, Deveaux V, Pechoux C, Chat S, Wang G, Vats N, Gendron F, Biomaterials, 32, 3988 (2011)

[8] Ra JC, Shin IS, Kim SH, Kang SK, Kang BC, Lee HY, Kim YJ, Jo JY, Yoon EJ, Choi HJ, Stem Cells Dev., 20, 1297 (2011)

[9] Ramakrishna S, Mayer J, Wintermantel E, Leong KE, Compos. Sci. Technol., 61, 1189 (2001)

[10] Pati F, Ha DH, Jang J, Han HH, Rhie JW, Cho DW, Biomaterials, 62, 164 (2015)

[11] Carrillo K, Tamayo G, Donohue A, Kobayashi T, Acuna R, J. Tissue Sci. Eng., 6, 2 (2015)

[12] Kim S, Shin BH, Yang C, Jeong S, Shim JH, Park MH, Choy YB, Heo CY, Lee K, Polymers, 10, 772 (2018)

[13] Kim WS, Mooney DJ, Arany PR, Lee K, Huebsch N, Kim J, Tissue Eng. A, 18, 737 (2012)

[14] Tan H, Ramirez CM, Miljkovic N, Li H, Rubin JP, Marra KGJB, Biomaterials, 30, 6844 (2009)

[15] Lee HW, Pyo SJCB, Chem. Biol. Interact., 298, 24 (2019)

[16] Patrick C, Chauvin P, Hobley J, Reece GPJTE, Reece, Tissue Eng., 5, 139 (1999)

[17] Liu S, Zhang H, Zhang X, Lu W, Huang X, Xie H, Zhou J, Wang W, Zhang Y, Liu Y, Tissue Eng. A, 17, 725 (2011)

[18] Moya ML, Cheng MH, Huang JJ, Francis-Sedlak ME, Kao SW, Opara EC, Brey EMJB, Biomaterials, 31, 2816 (2010)

[19] Choi JS, Yang HJ, Kim BS, Kim JD, Kim JY, Yoo B, Park K, Lee HY, Cho YW, J. Control. Release, 139, 2 (2009)

[20] Itoi Y, Takatori M, Hyakusoku H, Mizuno HJJ, J. Plast. Reconstr. Aesthet. Surg., 63, 858 (2010)

[21] Kim JS, Choi JS, Cho YW, Appl. Mater. Interfaces, 9, 8581 (2017)

[22] Shukla L, Yuan Y, Shayan R, Greening DW, Karnezis T, Front. Pharmacol., 11, 158 (2020)

[23] Gloeckner H, Jonuleit T, Lemke HD, J. Immunol. Methods, 252, 131 (2001)

[24] Yoshimura K, Suga H, Eto H, Regen. Med. (2009).

[25] Yoshimura K, Eto H, Kato H, Doi K, Aoi NJRM, Am. J. Clin. Pathol., 6, 33 (2011)

[26] Massensini AR, Ghuman H, Saldin LT, Medberry CJ, Keane TJ, Nicholls FJ, Velankar SS, Badylak SF, Modo M, Acta Biomater., 27, 116 (2015)

[27] Wen H, Xiao W, Biswas S, Cong ZQ, Liu XM, Lam KS, Liao YH, Deng W, ACS Appl. Mater. Interfaces., 11, 5821 (2019)

[28] Chen CY, Ke CJ, Yen KC, Hsieh HC, Sun JS, Lin FH, Theranostics, 5, 643 (2015)

[29] Hoareau L, Bencharif K, Girard AC, Gence L, Delarue P, Hulard O, Festy F, Roche R, J. Plast. Reconstr. Aesthet. Surg., 66, 712 (2013)

[30] Rajah R, Katz L, Nunn S, Solberg P, Beers T, Cohen P, Prog. Growth Factor Res., 6, 273 (1995)

[31] Gude MF, Frystyk J, Flyvbjerg A, Bruun JM, Richelsen B, Pedersen SBJGH, Growth Horm. IGF Res., 22, 200 (2012)

[32] Rajaram S, Baylink DJ, Mohan S, Endocr. Rev., 18, 801 (1997)

[33] Heinrich PC, Behrmann I, Haan S, Hermanns HM, Muller-Newen G, Schaper F, Biochem J., 374, 1 (2003)

[34] Hirano T, Taga T, Matsuda T, Hibi M, Suematsu S, Tang B, Murakami M, Kishimoto T, J. Cell Cloning, 8, 155 (1990)

[35] Bastard JP, Jardel C, Delattre J, Hainque B, Bruckert E, Oberlin F, Circulation, 99, 2219 (1999)

[36] Liu S, Zhang H, Zhang X, Lu W, Huang X, Xie H, Zhou J, Wang W, Zhang Y, Liu Y, Dneg Z, Jin Y, Tissue Eng. A., 17, 725 (2011)

[37] Armstrong R, Harrower T, Hurelbrink C, McLaughin M, Ratcliffe E, Tyers P, Richards A, Dunnett S, Rosser AE, Barker RJN, Neuroscience, 106, 201 (2001)

[38] Hogan P, Duplock L, Green M, Smith S, Gall KL, Frazer IH, O’Brien MF, Surgery C, J. Thorac., 112, 1260 (1996)

[39] Eto H, Kato H, Suga H, Aoi N, Doi K, Kuno S, Yoshimura K, Plast. Reconstr. Surg., 129, 1081 (2012)

[40] Carpaneda CA, Ribeiro MT, Aesth. Plast. Surg., 17, 43 (1993)

[41] Urs S, Smith C, Campbell B, Saxton AM, Taylor J, Zhang B, Snoddy J, Jones Voy B, Moustaid-Moussa N, J. Nutr., 134, 762 (2004)

[42] Ghaben AL, Scherer PE, Nat. Rev. Mol. Cell Biol., 20, 242 (2019)

[43] Makowski L, Brittingham KC, Reynolds JM, Suttles J, Hotamisligil GS, J. Biol. Chem., 280, 12888 (2005)

[44] Otero M, Lago R, Lago F, Casanueva FF, Dieguez C, Gomez-Reino J, Gualillo O, Febs Lett., 579(2), 295 (2005)