화학공학소재연구정보센터
홈 로그인 로그아웃 연락처 사이트맵
Journal of Industrial and Engineering Chemistry, Vol.96, 345-355, April, 2021
DOI10.1016/j.jiec.2021.01.041  Export Citation
Development of bioresorbable smart injectable hydrogels based on thermo-responsive copolymer integrated bovine serum albumin bioconjugates for accelerated healing of excisional wounds
V. H. Giang Phan1, Thai Minh Duy Le2, 3, Gopinathan Janarthanan4, Phuong-Khanh Thi Ngo1, Doo Sung Lee2, †, and Thavasyappan Thambi2, 3, †
  • 1Biomaterials and Nanotechnology Research Group, Faculty of Applied Sciences, Ton Duc Thang University, Ho Chi Minh City 70000, Vietnam
  • 2School of Chemical Engineering, Theranostic Macromolecules Research Center, Sungkyunkwan University, Suwon 16419, Republic of Korea
  • 3Department of Bioengineering, Hanyang University, Seoul 04763, Republic of Korea
  • 4Department of Chemical and Biomolecular Engineering, Seoul National University of Science and Technology, Seoul 01811, Republic of Korea
E-mail:,
One of the major challenges in wound healing is the development of suitable hydrogels that are injectable, biocompatible with multiple functionalities and properties such as high mechanical, tissue adhesiveness, and swelling properties. However, these hydrogels should not elicit any immunological response and synthesis steps should be easier and tunable according to the requirements. Considering these properties, we synthesized a thermo-sensitive triblock copolymer consisting of bovine serum albumin (BSA) protein capable of leveraging the needs for a proper wound closure and tissue regeneration on excisional injuries. Firstly, the triblock copolymer consisting of poly(e-caprolactone-colactide)-b-poly(ethylene glycol)-b-poly(e-caprolactone-co-lactide (PCLA) was synthesized and then the copolymer was grafted with BSA to yield BSA-PCLA bioconjugates. Aqueous solutions of free-flowing bioconjugate sol at room temperature can transform to gel at physiological temperature with high viscoelastic properties. Subcutaneous injection of BSA-PCLA bioconjugate sol into the back of Sprague-Dawley rats formed gel immediately and found to be bioresorbable after 5 weeks without significant toxicity at implantation sites. BSA-PCLA bioconjugate gel exhibited good adhesive property to major organs including liver, heart, and spleen when compared with control PCLA gel. When tested for in vivo wound closure trials, the BSA-PCLA gels showed rapid wound contraction compared to the PCLA and the control. The increased angiogenesis and collagen deposition were confirmed from the histological studies of the samples. These highly adhesive, biocompatible, biodegradable, thermos-sensitive bioconjugate gels show promising potential in wound healing and tissue regeneration without any additional biofactors or inorganic nanoparticles.
Keywords:Injectable hydrogels;Biodegradable copolymers;Thermo-responsive;Cutaneous wound;Tissue regeneration
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