The real sand is usually idealized by using upscaled particles, due to the large number of particles of tire-sand interaction. This study aims to determine a unique and complete set of DEM-FEM model parameters to improve numerical accuracy of tire-sand interaction after particles idealization. To achieve this aim, a novel method based on experimental design is proposed to calibrate the DEM-FEM model parameters by a series of single-factor numerical calibration tests. Initially, the interaction properties such as equivalent friction coefficients of particle-particle, particle-soil bin and particle-tire are determined successively by comparing experimental test with numerical simulation using the angle of repose as a bulk response. The material parameters of particles are then obtained by modified iteratively to match the stress-strain behavior of the granular assembly in triaxial test. After that, the calibrated parameter set is used to investigate the interaction mechanisms between the off-road tire and the granular terrain. Finally, the simulation results are qualitatively in agreement with the soil bin experiments, which verifies the effectiveness of the calibrated parameter set for the tractive performance analysis of tire-sand interaction. (C) 2020 The Society of Powder Technology Japan. Published by Elsevier B.V. and The Society of Powder Technology Japan. All rights reserved.