Light management strategy can be used to improve algal biomass and nutrient production. However, the response of algal metabolism to different light qualities, especially their interaction with other environmental factors, is not well understood. This study focuses on the interactive effects of light quality and culturing temperature on algal protein content and carbohydrate content of C. reinhardtii. Three LED light sources (blue light, red-orange light, and white-yellow light) were applied to grow algae in batch cultures with a light intensity of 105 mu mol/m(2)s under the temperatures of 24 degrees C to 32 degrees C. The protein and carbohydrate content were measured in both the late exponential growth phase and the late stationary growth phase. The results revealed that there was an interactive effect of light quality and culturing temperature on the protein and carbohydrate content. The combined conditions of blue light and a temperature of 24 degrees C or 28 degrees C, which induced a larger algal cell size with a prolonged cell cycle and a low division rate, resulted in the highest protein content; the protein mass fraction and concentration were 32% and 52% higher than that under white-yellow light at 32 degrees C. The combined conditions of red-orange light and a temperature of 24 degrees C, which promoted both the cell division and size growth, enhanced the carbohydrate content; the carbohydrate mass fraction and concentration were 161% and 155% higher than that under white-yellow light at 24 degrees C. When there was temperature stress (32 degrees C) or nutrient stress, the effect of light quality reduced, and the difference of protein and carbohydrate content among the three light qualities decreased.