In this study, the effects of the morphological characteristics of MoS2 nanomaterials on the glucose sensing of electrochemical biosensors were explored. Nanostructured MoS2 materials, including nanoparticles (NPs), nanoflowers (NFs), and nanoplatelets (NPLs), were prepared via a simple hydrothermal method. The structure and morphological characteristics of MoS2 nanomaterials were examined through X-ray diffraction, field emission scanning electron microscopy, and Raman spectroscopy. Electrochemical properties were analyzed through cyclic voltammetry. Results showed that the obtained sensitivity was 64, 68.7, and 77.6 mu AmM-1 cm(-2) for MoS2 NP-, MoS2 NF-, and MoS2 NPL-based biosensors, respectively. The limit of detection (LOD) of all MoS2-based glucose biosensors was 0.081 mM. In addition, the pH, temperature, glucose oxidase (GO(x)) concentration, reproducibility, specificity, and stability of glucose biosensors with different MoS2 morphologies were also investigated and indicated the oxidation current response of the MoS2 NPL-based glucose biosensor was higher than that of MoS2 NFand NP-based biosensors.