Even though organisms with squalene hopene cyclase activity involved in hopanoid synthesis has been reported earlier, their existence along with carotenoid synthesis is rarely reported. Here, we report the existence of hopanoid and C(30)carotenoid biosynthetic pathway inPseudomonas mendocina,the squalene hopene cyclase producing endophyte of the medicinal plantMurraya koenigii.The enzyme squalene hopene cyclase fromPseudomonas mendocinais involved in the synthesis of dehydrosqualene-mediated alternate pathway for carotenoid biosynthesis. The hopanoids are involved in membrane stability and integrity, and the carotene chromophores are involved in the photo protection of the cell. The orange-colored C(30)carotenoid pigment 4-4 ' diaponeurosporenic acid in the extracellular extract ofPseudomonas mendocinawith squalene cyclase activity was detected by the combination of UV/Vis spectrometry, FTIR, and Mass Spectrometry. 4-4 ' diaponeurosporenic acid could be traced as the end product of the carotenoid pathway and belonged to the xanthophyll group of carotenoids.