Biodegradation of pesticides by bacteria, fungi, algae, and other organisms is an eco-friendly, efficient, and economical method of detoxification. This study was conducted to evaluate the efficiency ofCandida tropicalisfor potential degradation of metalaxyl in the aqueous media. The yeast fungus from soil with a historical application of metalaxyl has been isolated. The identification of isolated fungus was carried out using 28S rRNA. The effect of pH, temperature, and metalaxyl concentration on the growth ofC. tropicalisand its ability for metalaxyl degradation was also investigated. The isolated fungus was identified asC. tropicalisbased on the sequencing of D1D2 region of the 28S rRNA and BLAST search of the nucleotide sequences. The growth ofC. tropicalisin the presence of metalaxyl was significantly higher than in the absence of it. A temperature of 30 degrees C, a pH value of 7, and metalaxyl concentration of 5 mg/L were the optimal conditions forC. tropicalisto grow and to obtain the highest degradation rate of metalaxyl. At the optimum conditions, the degradation rate and the half-life value of metalaxyl were 0.065 d-1 and 10.7 days, respectively. Under the optimum conditions, a 98% degradation of metalaxyl initial concentration obtained within 36 days. This report is novel in showing the biodegradation of metalaxyl in water by the yeastC. tropicalis, which can be a promising method in this regard.