A Gram-stain-negative, catalase- and oxidase-positive, rod-shaped and motile strain FT127W(T)was isolated from a subtropical stream in China. Comparison based on 16S rRNA gene sequences showed that strain FT127W(T)belongs to genusMassiliaand shares 98.5% similarity withMassilia buxeaA9(T)as its closest neighbor. The genome size of strain FT127W(T)was 6.65 Mbp with G + C content of 65.3%. The calculated pairwise OrthoANIu values and digital DNA-DNA hybridization values between strain FT127W(T)and each of strainsM. buxeaKCTC 52429(T),Massilia armeniacaZMN-3(T),Massilia plicataDSM 17505(T)andMassilia namucuonensisCGMCC 1.11014(T)were less than 83.1% and 26.6%, respectively. The reconstructed phylogenomic tree based on concatenated 92 core genes showed that strain FT127W(T)clusters closely withM. namucuonensisCGMCC 1.11014(T). The respiratory quinone of strain FT127W(T)was Q-8. The major fatty acids were C-16:1 omega 7c, C(16:0)and C-12:0. The polar lipids consisted of phosphatidylethanolamine, phosphatidylglycerol and one unidentified phospholipid. Combining above all characteristics, strain FT127W(T)should represent a novel species within genusMassilia, for which the nameMassilia aquaticasp. nov. (type strain FT127W(T) = GDMCC 1.1690(T) = KACC 21482(T)) is proposed.