To boost the energy density as well as address the safety issues, an effective strategy is to replace the liquid electrolytes with solid-state electrolytes (SSEs). However, the current SSEs usually show low ionic conductivity at room temperature and large interfacial impedance with electrodes, which hinders the operation of the all-solid-state lithium batteries (ASSLBs) at room temperature. In this Review, we summarize the recent progress related the fundamental properties, preparation techniques, and electrochemical performance of room-temperature ASSLBs using different types of SSEs, such as polymer-based, oxides-based, and sulfides-based SSEs. We analyze the progress in resolving the issues associated with the low ionic conductivity of polymer-based SSEs and high interfacial resistance and instability of inorganic SSEs. Furthermore, the key issues and critical perspectives to develop high-performance ASSLBs at room temperature are discussed.