The present study involves obtaining biodiesel from freshwater algae biomass. Biodiesel is prepared using the transesterification method. Three types of biodiesel samples are produced using three different alcohols (methanol/ethanol/butanol). The prepared biodiesel samples are tested for their properties by adopting American Society for Testing and Materials (ASTM) standards. A B20 blend of these esters is examined in a compression ignition engine for determining its performance as well as emission characteristics. The results confirmed that the brake thermal efficiency (BTE) values of the biodiesel blends were closer to that of diesel, and its brake specific consumption values were higher. There was a considerable reduction in emissions of CO and UHC for biodiesel blends; their NO(x )emission values are slightly higher than that of diesel. Out of three esters used, methyl esters show improved performance and emission characteristics.