The biofuel capacity of the plant obtained through hybridization (tekin-2017) was explored in this report. Firstly, hydro-distillation method was used to extract oil. And transformed by transesterification into Biofuel. Biofuel was mixed in 5% (HB-5) volume with diesel fuel. On a four-stroke diesel generator engine, HB-5 fuel and reference diesel (Df) fuel were tested. The tests were conducted at constant engine speed (1500) and at various loads (3.52-7.04-10.56 kW). HB-5 fuel reduced Carbon Monoxide (CO) and Hydrocarbon (HC) emissions while increasing Azotoxide emission (NOx).In the data of combustion. Cumulative heat release (CHR), mass-burn rate (MFR), average gas temperature (AGT), and heat release rate (HRR) decreased for HB-5 fuel. Cylinder pressure (CP) and density of Knock (KD) increased.