The low critical solution temperature (LCST) and minimum salt concentration that cause phase splitting in water and short-chain alcohol mixtures were determined experimentally. The short-chain alcohols, which are completely miscible with water (methanol, ethanol, 1-propanol, 2-propanol, and tert-butanol) and most of the common salts (those with Na+, K+, Ca2+, and NH4+ cations and Cl-, SO42-, CO32-, and NO3- anions), were studied. Experimentally determined data increased future development opportunities of column sequences and process design using aqueous two-phase systems (ATPSs) by establishing alcohols, some salts, and their operational limits. An example using these systems in phase transition extraction (PTE) separation processes was proposed and discussed. Finally, the use of different thermodynamic models, such as the extended UNIQUAC for electrolytes and the electrolyte nonrandom two-liquid (NRTL) model, for equilibrium calculations and the LCSTs necessary for simulation of these extraction processes with ATPS systems were analyzed.