Skin-like flexible membrane with excellent water resistance and moisture permeability is an urgent need in the wound dressing field to provide comfort and protection for the wound site. Despite efforts that have been made in the development of waterproof and breathable (W&B) membranes, the in-situ electrospinning of W&B membranes suitable for irregular wound surfaces as wound dressings still faces huge challenges. In the current work, a portable electrospinning device with multi-functions, including adjustable perfusion speed for a large range from 0.05 mL/h to 10 mL/h and high voltage up to 11 kV, was designed. The thymol-loaded ethanol-soluble polyurethane (EPU) skin-like W&B nanofibrous membranes with antibacterial activity were fabricated via the custom-designed device. Ultimately, the resultant nanofibrous membranes composed of EPU, fluorinated polyurethane (FPU), and thymol presented uniform structure, robust waterproofness with the hydrostatic pressure of 17.6 cm H2O, excellent of 3.56 kg m(-2) d(-1), the high tensile stress of 1.83 MPa and tensile strain of 453%, as well as high antibacterial activity. These results demonstrate that the new-type device has potential as a portable electrospinning apparatus for the fabrication of antibacterial membranes directly on the wound surface and puts a new way for the development of portable electrospinning devices. (C) 2021 Elsevier Inc. All rights reserved.